Univ Lyon, Université Lyon 1, Inserm, INRAE, Stem Cell and Brain Research Institute, U1208, USC1361, F-69500, Bron, France.
Univ Lyon, Université Lyon 1, Inserm, INRAE, Stem Cell and Brain Research Institute, U1208, USC1361, F-69500, Bron, France.
Exp Cell Res. 2020 Apr 15;389(2):111908. doi: 10.1016/j.yexcr.2020.111908. Epub 2020 Feb 11.
Both embryo-derived (ESC) and induced pluripotent stem cell (iPSC) lines have been established in rabbit. They exhibit the essential characteristics of primed pluripotency. In this review, we described their characteristic features at both molecular and functional levels. We also described the attempts to reprogram rabbit pluripotent stem cells (rbPSCs) toward the naive state of pluripotency using methods established previously to capture this state in rodents and primates. In the last section, we described and discussed our current knowledge of rabbit embryo development pertaining to the mechanisms of early lineage segregation. We argued that the molecular signature of naive-state pluripotency differs between mice and rabbits. We finally discussed some of the key issues to be addressed for capturing the naive state in rbPSCs, including the generation of embryo/PSC chimeras.
胚胎衍生的（ESC）和诱导多能干细胞（iPSC）系已在兔中建立。它们表现出初始多能性的基本特征。在这篇综述中，我们描述了它们在分子和功能水平上的特征。我们还描述了使用先前在啮齿动物和灵长类动物中建立的方法，尝试将兔多能干细胞（rbPSC）重编程为原始多能性状态的尝试。在最后一节中，我们描述并讨论了我们目前对与早期谱系分离机制相关的兔胚胎发育的了解。我们认为，原始状态多能性的分子特征在小鼠和兔子之间存在差异。最后，我们讨论了在 rbPSC 中捕获原始状态需要解决的一些关键问题，包括胚胎/PSC 嵌合体的产生。
