在摩洛哥硬皮病伴抗着丝点自身抗体患者中 CENP-B 表位的蛋白质组学特征。

Proteomics characterization of CENP-B epitope in Moroccan scleroderma patients with anti-centromere autoantibodies.

机构信息

Biomedical Genomics and Oncogenetics Research Laboratory, Department of Biology, Faculty of Sciences and Techniques of Tangier, Abdelmalek Essaadi University, Morocco.

Biomedical Genomics and Oncogenetics Research Laboratory, Department of Biology, Faculty of Sciences and Techniques of Tangier, Abdelmalek Essaadi University, Morocco; Department of Biomedicine, Biotechnology and Public Health, Faculty of Sciences, University of Cadiz, Cadiz, Spain.

出版信息

Immunol Lett. 2020 May;221:1-5. doi: 10.1016/j.imlet.2020.02.005. Epub 2020 Feb 11.

DOI:10.1016/j.imlet.2020.02.005

PMID:32057908

Abstract

BACKGROUND

Anti-centromere auto-antibodies (ACA) have been described as a marker in Systemic sclerosis (SSc) disease. CENP-B is the major centromere auto-antigen recognized by SSc patients with positive ACA. Our aim was to characterize the major epitope involved in the anti-CENP-B immune response of Moroccan SSc patients.

PATIENTS AND METHOD

For identification of SSc biomarkers, 80 sera from patients with SSc and systemic lupus erythematosus (SLE) were screened by indirect immunofluorescence test (IIF) to assess the presence of ANA reactivity. Immunoblotting analysis was performed for 11 sera with positive ACA using the N-terminal and C-terminal region of CENP-B protein as antigens.

RESULTS

29 out of 30 (96, 66 %) patients with SSc had positive ANA. 11 out of 30 (36, 67 %) patients were ACA positive and 6 of them produced auto-antibodies against Nt-CENPB antigen. Two of these 6 Nt-CENPB positive sera produced also other auto-antibodies associated to primary biliary cirrhosis. None of all sera tested showed reactivity against Ct-CENPB.

CONCLUSION

Our data showed, for the first time in Morocco, that the Nt-CENPB contains a major epitope for Moroccan SSc patients. These findings could provide additional information that would contribute to improving the diagnosis and management of these patients.

摘要

背景

抗着丝点自身抗体（ACA）已被描述为系统性硬化症（SSc）疾病的标志物。CENP-B 是 SSc 患者阳性 ACA 识别的主要着丝粒自身抗原。我们的目的是鉴定摩洛哥 SSc 患者抗 CENP-B 免疫反应中涉及的主要表位。

患者和方法

为了鉴定 SSc 生物标志物，通过间接免疫荧光试验（IIF）筛选了 80 份来自 SSc 和系统性红斑狼疮（SLE）患者的血清，以评估 ANA 反应性的存在。使用 CENP-B 蛋白的 N 端和 C 端区域作为抗原，对 11 份具有阳性 ACA 的血清进行免疫印迹分析。

结果

30 名 SSc 患者中有 29 名（96%，66%）ANA 阳性。11 名 SSc 患者中有 11 名（36%，67%）ACA 阳性，其中 6 名产生针对 Nt-CENPB 抗原的自身抗体。这 6 份 Nt-CENPB 阳性血清中的 2 份也产生了与原发性胆汁性肝硬化相关的其他自身抗体。所有测试的血清均未显示与 Ct-CENPB 的反应性。