College of Architecture and Environment, Sichuan University, Chengdu 610065, China; CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
College of Architecture and Environment, Sichuan University, Chengdu 610065, China.
Bioresour Technol. 2020 May;303:122949. doi: 10.1016/j.biortech.2020.122949. Epub 2020 Feb 4.
Reduction in water consumption and increase in ethanol concentration are two main challenges for bioethanol production from lignocellulosic materials. To address the two challenges, the aim of this work was to study the production of bioethanol from unwashed-pretreated rapeseed straw (RS) at high solid loading. RS pretreated with 1% (w w) HSO at 160 °C for 10 min resulted in excellent digestibility and fermentability of pretreated RS. The unwashed-pretreated RS was subjected to presaccharification and fed-batch simultaneous saccharification and fermentation (P-FB-SSF) at a final solid loading of 22% (w w). Ethanol concentration and ethanol yield of 53.1 g L (equivalent to 4.1% (w w) based on fermentation slurry) and 72.4% were obtained, respectively. In total, 92.1 g water g ethanol was consumed, a much smaller amount than that observed with washing after pretreatment or fermentation performed at lower solid loading.
减少耗水量和提高乙醇浓度是从木质纤维素原料生产生物乙醇的两个主要挑战。为了解决这两个挑战，本工作旨在研究在高固体负荷下从不经水洗预处理的油菜秸秆（RS）生产生物乙醇。用 1%（w w）的 HSO 在 160°C 下预处理 10 分钟的 RS 具有极好的可消化性和可发酵性。未经水洗预处理的 RS 进行预糖化和分批补料同时糖化和发酵（P-FB-SSF），最终固体负荷为 22%（w w）。乙醇浓度和乙醇得率分别为 53.1 g L（基于发酵浆计为 4.1%（w w））和 72.4%。总的来说，消耗了 92.1 g 水 g 乙醇，比预处理后水洗或在较低固体负荷下发酵时观察到的要少得多。
