Avian Disease and Oncology Laboratory, Agriculture Research Service, United States Department of Agriculture, East Lansing, MI, USA.
Institute of Special Wild Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, No 4899, Jv Ye Street, Changchun, Jilin 130112, China.
Genomics. 2020 May;112(3):2564-2571. doi: 10.1016/j.ygeno.2020.02.009. Epub 2020 Feb 12.
Marek's disease (MD) is a lymphoproliferative disease of domestic chickens caused by a cell-associated oncogenic alpha-herpesvirus, Marek's disease virus (MDV). Clinical signs of MD include bursal/thymic atrophy, neurologic disorders, and T cell lymphomas. MiRNAs play key roles in regulation of gene expression by targeting translational suppression or mRNA degradation. MDV encodes miRNAs that are associated with viral pathogenicity and oncogenesis. In this study, we performed miRNA sequencing in the bursal tissues, non-tumorous but viral-induced atrophied lymphoid organ, from control and infected MD-resistant and susceptible chickens at 21 days post infection. In addition to some known miRNAs, a minimum of 300 novel miRNAs were identified in each group that mapped to the chicken genome with no sequence homology to existing miRNAs in chicken miRbase. Comparative analysis identified 54 deferentially expressed miRNAs between the chicken lines that might shed light on underlying mechanism of bursal atrophy and resistance or susceptibility to MD.
马立克氏病（MD）是一种由细胞相关的致瘤性α疱疹病毒，马立克氏病病毒（MDV）引起的家禽淋巴组织增生性疾病。MD 的临床症状包括法氏囊/胸腺萎缩、神经紊乱和 T 细胞淋巴瘤。miRNAs 通过靶向翻译抑制或 mRNA 降解在调节基因表达中发挥关键作用。MDV 编码与病毒致病性和致癌性相关的 miRNAs。在这项研究中，我们在感染后 21 天的抗 MD 抗性和易感鸡的非肿瘤但病毒诱导的萎缩淋巴器官的法氏囊中进行了 miRNA 测序。除了一些已知的 miRNAs 之外，在每个组中还鉴定出至少 300 种新的 miRNA，这些 miRNA 映射到鸡基因组上，与鸡 miRbase 中的现有 miRNA 没有序列同源性。比较分析鉴定了鸡系之间 54 种差异表达的 miRNAs，这可能有助于阐明法氏囊萎缩以及对 MD 的抗性或易感性的潜在机制。
