College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
Vet Clin Pathol. 2020 Mar;49(1):17-22. doi: 10.1111/vcp.12823. Epub 2020 Feb 14.
In 2015, a previously unrecognized intracytoplasmic erythrocytic inclusion was discovered in anemic wild-caught adult gopher tortoises (Gopherus polyphemus). Subsequently, molecular diagnostics revealed this inclusion to be a novel Anaplasma sp.
The goal of this study was to morphologically characterize these erythrocytic inclusions by light and transmission electron microscopy (TEM).
Blood samples were taken from two car-injured wild-caught gopher tortoises for the preparation of Wright-Giemsa stained smears and TEM specimens. CBC data were serially performed and morphologically examined during treatment periods.
Studies revealed a moderate to severe anemia with moderate regeneration as indicated by polychromasia and the presence of immature erythroid precursors. In addition, on light microscopy, one to two variably-sized round basophilic stippled paracentral erythrocytic inclusions were present per cell in both animals and involved 10%-25% of erythrocytes. TEM identified the intraerythrocytic inclusions as discrete membrane-bound cytoplasmic vacuoles (morulae) containing membrane-bound bacterial subunits that were of variable size, shape, and electron density. Serial hematologic data indicated complete remission of the infection in response to a single long-term course of doxycycline.
The presence of a regenerative anemia in gopher tortoises from Florida revealed a newly recognized bacterial species that has morphologic characteristics similar to members of the genus Anaplasma.
2015 年，在患有贫血症的野生捕获成年地鼠龟（Gopherus polyphemus）中发现了一种以前未被识别的细胞质内红细胞内含物。随后，分子诊断学显示这种内含物是一种新型的无形体属。
本研究的目的是通过光镜和透射电子显微镜（TEM）对这些红细胞内含物进行形态学特征描述。
从两辆汽车受伤的野生捕获的地鼠龟中抽取血液样本，用于制备 Wright-Giemsa 染色涂片和 TEM 标本。在治疗期间，连续进行 CBC 数据并进行形态学检查。
研究表明，存在中度至重度贫血症，伴有中度再生，表现为多色性和存在未成熟的红系前体细胞。此外，在光镜下，在两个动物的每个细胞中存在一个到两个大小不等的圆形嗜碱性点状中央旁红细胞内含物，占红细胞的 10%-25%。TEM 鉴定出红细胞内包含物为离散的膜结合细胞质空泡（桑葚体），其中包含膜结合的细菌亚单位，其大小、形状和电子密度各不相同。连续的血液学数据表明，感染在单次长期多西环素治疗后完全缓解。
在佛罗里达州的地鼠龟中出现再生性贫血症表明存在一种新的细菌物种，其形态特征与无形体属的成员相似。
