Institute of Nuclear Agricultural Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhorn Pathom 73170, Thailand.
Institute of Nuclear Agricultural Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
Int J Biol Macromol. 2020 May 1;150:831-838. doi: 10.1016/j.ijbiomac.2020.02.119. Epub 2020 Feb 19.
Genetic diversity in the physicochemical properties and fine structures of seven cassava starches samples was studied. The apparent amylose content ranged from 24.8 to 27.6%. The whole branched starches showed significant differences in average hydrodynamic radius, ranging from 53.35 to 58.45 nm, while debranched starch exhibited differences in degrees of polymerization and height of both amylose and amylopectin peaks. The molecular size of amylose and amylopectin was positively correlated. The amount of short chains fa (6 ≤ X ≤ 12) and fb1 (13 ≤ X ≤ 24) had significant differences among the cultivars. Structure-function relation analysis indicated that the CPV and SB were mainly determined by amylopectin fine structures, BD, PTi and Tp and retrogradation properties were mainly determined by the amylose fine structure, while PTe and To were mainly affected by both amylose and amylopectin fine structures. The current findings will be helpful to improve the understanding cassava starch quality for use in industrial starch applications.
研究了 7 个木薯淀粉样品的理化性质和精细结构的遗传多样性。直链淀粉含量在 24.8%至 27.6%之间。整个支链淀粉的平均流体力学半径存在显著差异，范围在 53.35 至 58.45nm 之间，而去支链淀粉在聚合度和直链淀粉和支链淀粉峰的高度上存在差异。直链淀粉和支链淀粉的分子量呈正相关。短链 fa（6≤X≤12）和 fb1（13≤X≤24）的含量在品种间存在显著差异。结构-功能关系分析表明，CPV 和 SB 主要由支链淀粉精细结构决定，BD、PTi 和 Tp 以及回生特性主要由直链淀粉精细结构决定，而 PTe 和 To 主要受直链淀粉和支链淀粉精细结构的影响。本研究结果将有助于提高对工业淀粉应用中木薯淀粉质量的认识。
