Institute of Blood Transfusion, Peking Union Medical College and Chinese Academy of Medical Sciences, No.26 Huacai Road, Chenghua District, Chengdu, China.
Department of Blood Transfusion, Chinese PLA General Hospital, No.28 Fuxing Road, Haidian District, Beijing, China.
J Photochem Photobiol B. 2020 Mar;204:111782. doi: 10.1016/j.jphotobiol.2020.111782. Epub 2020 Feb 5.
Ultraviolet (UV) fluorescent lamp (FL) was applied in mainstream riboflavin photochemical method (RPM) to inactivate pathogens in blood components. Low UV irradiance emitted by UV-FL resulted in more time to achieve effective inactivation.
A novel light emitting diode (LED) UV illumination with adjustable irradiance was developed by us. Two strains of drug-resistant bacteria (DRB), pan-drug resistant Acinetobacter baumannii (PDRAB) and methicillin-resistant Staphylococcus aureus (MRSA) were cultured and used for evaluating the inactivation effectiveness of RPM using UV-LED or UV-FL against DRB in plasma or platelets. Three plasma factors and four platelet parameters were measured after treatments.
There was a linear relationship between UV-LED irradiance and electric current, the minimum UV irradiance was 24 mW/cm, and the maximum was 258 mW/cm. At the same UV dose of 15 J/cm, inactivation effectiveness of UV-LED with 258 mW/cm against PDRAB in plasma or platelets were comparable to that of UV-FL with 16 mW/cm, both above 98%. UV-FL treatment required 10-15 min, but UV-LED only required 1-2 min. However, MRSA showed a resistance to UV-LED (inactivation effectiveness was around 40%) compared with UV-FL (inactivation effectiveness was above 98%). The retention of fibrinogen, factor V, factor VII in plasma and platelet counts in platelets with UV-LED treatment were significantly higher than UV-FL at the same UV dose.
The treatment of RPM using UV-LED with high UV irradiance was able to dramatically shorten inactivation time against PDRAB in plasma or platelets and improve retention of blood components compared with UV-FL.
紫外线（UV）荧光灯（FL）被应用于主流核黄素光化学方法（RPM）以灭活血液成分中的病原体。UV-FL 发出的低 UV 辐照度导致需要更多时间来实现有效灭活。
我们开发了一种具有可调辐照度的新型发光二极管（LED）UV 照明。两种耐药菌（DRB），泛耐药鲍曼不动杆菌（PDRAB）和耐甲氧西林金黄色葡萄球菌（MRSA）被培养并用于评估 RPM 使用 UV-LED 或 UV-FL 对血浆或血小板中 DRB 的灭活效果。处理后测量了三个血浆因子和四个血小板参数。
UV-LED 辐照度与电流之间存在线性关系，最小 UV 辐照度为 24 mW/cm，最大辐照度为 258 mW/cm。在相同的 15 J/cm UV 剂量下，258 mW/cm 的 UV-LED 对血浆或血小板中 PDRAB 的灭活效果与 16 mW/cm 的 UV-FL 相当，均超过 98%。UV-FL 处理需要 10-15 分钟，但 UV-LED 只需 1-2 分钟。然而，MRSA 对 UV-LED 表现出耐药性（灭活效果约为 40%），而对 UV-FL 的灭活效果超过 98%。与 UV-FL 相比，UV-LED 处理在相同的 UV 剂量下可显著提高血浆中纤维蛋白原、因子 V、因子 VII 的保留率和血小板中的血小板计数。
与 UV-FL 相比，使用高 UV 辐照度的 UV-LED 处理 RPM 能够显著缩短血浆或血小板中 PDRAB 的灭活时间，并提高血液成分的保留率。
