Tao Ruiyang, Zhang Jingyi, Xia Ruocheng, Yang Zihao, Wang Shouyu, Zhang Xiaochun, Yang Qi, Zhang Suhua, Li Chengtao
Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P.R. China.
Institute of Forensic Medicine, West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu, P.R. China.
Ann Hum Biol. 2020 Feb;47(1):59-64. doi: 10.1080/03014460.2019.1704871.
The value of using X-chromosomal short tandem repeats (X-STRs) as genetic markers in human genetics has been widely recognised. However, the 16 X-STRs in the Goldeneye DNA ID System 17X kit have not been thoroughly applied. To investigate the genetic polymorphisms of 16 X-STRs in three main ethnic minorities (Tibetan, Mongolian and Kazakh) in China and to reveal the phylogenetic relationships of different populations. A total of 245 Tibetan, 168 Mongolian and 105 Kazakh individuals were genotyped using this 17X kit. The allelic frequencies and other parameters were calculated. An additional eight Chinese populations and nine global populations were included in genetic comparisons based on 16 or 8 overlapped X-STRs. A total of 147 alleles were observed from 16 X-STRs with allelic frequencies ranging from 0.0024 to 0.7952 in the three studied groups. Based on 16 X-STRs, Tibetans, Kazakhs and Mongolians showed more similarity to each other and were genetically distinct from the Shanghai Han group; based on 8 X-STRs, only the genetic relationships between different nations could be clarified. Our study presents an extensive report on a novel X-STR assay in three Chinese ethnic groups and a comprehensive genetic comparison between different populations based on these X-STRs.
在人类遗传学中，使用X染色体短串联重复序列（X-STRs）作为遗传标记的价值已得到广泛认可。然而，Goldeneye DNA ID System 17X试剂盒中的16个X-STRs尚未得到充分应用。为了研究中国三个主要少数民族（藏族、蒙古族和哈萨克族）中16个X-STRs的遗传多态性，并揭示不同群体的系统发育关系。使用该17X试剂盒对总共245名藏族、168名蒙古族和105名哈萨克族个体进行基因分型。计算等位基因频率和其他参数。基于16个或8个重叠的X-STRs，将另外8个中国群体和9个全球群体纳入遗传比较。在三个研究组中，从16个X-STRs中共观察到147个等位基因，等位基因频率范围为0.0024至0.7952。基于16个X-STRs，藏族、哈萨克族和蒙古族彼此之间表现出更多的相似性，并且在基因上与上海汉族群体不同；基于8个X-STRs，只能阐明不同民族之间的遗传关系。我们的研究提供了一份关于中国三个民族中一种新型X-STR检测方法的广泛报告，以及基于这些X-STRs对不同群体进行的全面遗传比较。
