Department of Endocrinology and Metabolism, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Laboratory of Endocrinology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Horm Metab Res. 2020 Feb;52(2):109-116. doi: 10.1055/a-1082-1080. Epub 2020 Feb 13.
Mesencephalic astrocyte-derived neurotrophic factor (MANF) is a novel neurotrophic factor. Although recent studies have suggested that MANF appeared to be associated with insulin resistance, the results have been inconsistent. The aim of our study was to determine the serum MANF levels in women with PCOS and controls, to investigate their relationship to insulin resistance, and to evaluate circulating MANF changes with metformin intervention in PCOS women. We conducted a series of cross-sectional and interventional studies in 90 newly diagnosed patients with PCOS and 60 age- and gender-matched controls. Oral glucose tolerance test and euglycemic-hyperinsulinemic clamps were performed to assess the glucose tolerance and insulin sensitivity. Forty-three women with PCOS were randomly assigned to six months of oral metformin therapy. Serum MANF levels were significantly lower in women with PCOS than in controls. Serum MANF levels were positively correlated with M-value and negatively correlated with body mass index (BMI), body fat percentage (FAT), homeostatic model assessment of insulin resistance (HOMA-IR), and free androgen index (FAI). Multivariate stepwise regression demonstrated that serum MANF levels were independently associated with M-value and FAI. After six months of metformin treatment, there was a significant increase in serum MANF levels in PCOS women. Serum MANF levels are decreased in women with PCOS, and are reversely related to insulin resistance and hyperandrogenism. Metformin treatment elevates serum MANF levels and alleviates insulin resistance and hyperandrogenism in PCOS women.
中脑星形胶质细胞衍生神经营养因子（MANF）是一种新型的神经营养因子。虽然最近的研究表明，MANF 似乎与胰岛素抵抗有关，但结果并不一致。我们的研究目的是确定多囊卵巢综合征（PCOS）患者和对照组妇女的血清 MANF 水平，探讨其与胰岛素抵抗的关系，并评估二甲双胍干预对 PCOS 妇女循环 MANF 的变化。我们在 90 例新诊断的 PCOS 患者和 60 例年龄和性别匹配的对照组中进行了一系列横断面和干预研究。进行口服葡萄糖耐量试验和正常血糖高胰岛素钳夹试验以评估葡萄糖耐量和胰岛素敏感性。43 例 PCOS 妇女被随机分为接受 6 个月的口服二甲双胍治疗。与对照组相比，PCOS 妇女的血清 MANF 水平显著降低。血清 MANF 水平与 M 值呈正相关，与体重指数（BMI）、体脂百分比（FAT）、稳态模型评估的胰岛素抵抗（HOMA-IR）和游离雄激素指数（FAI）呈负相关。多元逐步回归分析表明，血清 MANF 水平与 M 值和 FAI 独立相关。经过 6 个月的二甲双胍治疗，PCOS 妇女的血清 MANF 水平显著升高。PCOS 妇女的血清 MANF 水平降低，与胰岛素抵抗和高雄激素血症呈负相关。二甲双胍治疗可升高血清 MANF 水平，并缓解 PCOS 妇女的胰岛素抵抗和高雄激素血症。
