School of Chemistry and Chemical Engineering, Analytical Testing Center, Institute of Micro&Nano Intelligent Sensing, Neijiang Normal University, Neijiang, 641100, PR China; Chemical Synthesis and Pollution Control Key Laboratory of Sichuan Province, China West Normal University, Nanchong, 637000, PR China.
School of Chemistry and Chemical Engineering, Analytical Testing Center, Institute of Micro&Nano Intelligent Sensing, Neijiang Normal University, Neijiang, 641100, PR China.
J Chromatogr A. 2020 May 24;1619:460935. doi: 10.1016/j.chroma.2020.460935. Epub 2020 Feb 9.
An enhanced fluorescence detection system of capillary electrophoresis (CE) was equipped with a concave silver mirror, by which the detection sensitivity of light-emitting diode induced fluorescence (LEDIF) can be increased greatly. The silver concave mirror and the cathode window in photomultiplier tube (PMT) were accurately set face to face at the same axis. When the two labeled tumor markers exactly moved to the center of detection window, the emission from analytes are excitated by LED source. Currently, the analytes may be regarded as a luminescent source point. When the source point exactly moves to the focus of the concave mirror, the emission of the labeled sample was collected effectively, enhanced by convergence and reflected by the concave mirror. Then it was sensitively detected by the PMT. The optical mechanism of enhancing detection sensitivity was explored. A simple comparative test on sensitivity was carried out, which aimed to compare sensitivity of the new detection system with concave mirror to that without concave mirror but the other conditions were kept the same. Two tumor markers labeled with FITC were selected for the test, using the simple LEDIF detect system. The results (LOD, 150 nM for L-Leu and L-Val) showed that the detection sensitivity matched with concave mirror reached more 16 times than the detection method without concave mirror.
一种增强型毛细管电泳（CE）荧光检测系统配备了凹面银反射镜，可大大提高发光二极管诱导荧光（LEDIF）的检测灵敏度。凹面银反射镜和光电倍增管（PMT）的阴极窗精确地设置在同一轴上，面对面。当两个标记的肿瘤标志物正好移动到检测窗口的中心时，LED 光源会激发分析物的发射。目前，分析物可以被视为一个发光源点。当源点正好移动到凹面镜的焦点时，标记样品的发射被有效收集，通过收敛增强，并由凹面镜反射。然后，它被 PMT 灵敏地检测。探讨了提高检测灵敏度的光学机制。进行了简单的灵敏度比较测试，旨在比较带有凹面镜的新检测系统与没有凹面镜但其他条件相同的系统的灵敏度。选择两种用 FITC 标记的肿瘤标志物进行测试，使用简单的 LEDIF 检测系统。结果（LOD，L-Leu 和 L-Val 的 150 nM）表明，带有凹面镜的检测灵敏度比没有凹面镜的检测方法提高了 16 倍以上。
