Benaki Phytopathological Institute, Department of Phytopathology, Laboratory of Virology, 14561 Kifissia, Greece.
Benaki Phytopathological Institute, Department of Pesticides Control and Phytopharmacy, 14561 Kifissia, Greece.
Virus Res. 2020 Apr 15;280:197899. doi: 10.1016/j.virusres.2020.197899. Epub 2020 Feb 14.
The Plasma membrane Cation binding Protein 1 (PCaP1) has been shown to be important for the intra-cellular movement of two members of the Potyvirus genus in arabidopsis and tobacco plants. In this study, the orthologous PCaP1 gene of pepper (Capsicum annuum) was examined for its role in the accumulation of Potato virus Y, type member of the Potyvirus. Downregulation of C. annuum PCaP (CaPCaP) through tobacco rattle virus-induced gene silencing, resulted in lower accumulation of potato virus Y (PVY) in pepper plants. Using an improved pepper protoplast isolation protocol, we showed that knockdown of CaPCaP negatively affected PVY accumulation at the within-cell level in pepper in contrast with the turnip mosaic virus-arabidopsis pathosystem. Conversely, following overexpression of CaPCaP, the accumulation of PVY at the systemic level was increased. The results provide further knowledge on the role of PCaP in the potyvirus infection process and reveal differences of its action among different pathosystems.
质膜阳离子结合蛋白 1（PCaP1）已被证明对马铃薯 Y 病毒（Potyvirus）属的两个成员在拟南芥和烟草植物中的细胞内运动很重要。在这项研究中，研究了辣椒（Capsicum annuum）的同源 PCaP1 基因在马铃薯 Y 病毒（Potato virus Y，PVY）积累中的作用，PVY 是 Potyvirus 的成员之一。通过烟草脆裂病毒诱导的基因沉默下调辣椒 C. annuum PCaP（CaPCaP）的表达，导致辣椒植物中马铃薯 Y 病毒（PVY）的积累降低。利用改良的辣椒原生质体分离方案，我们表明，与芜菁花叶病毒-拟南芥病理系统相比，CaPCaP 的敲低会在辣椒细胞内水平上对 PVY 积累产生负面影响。相反，过表达 CaPCaP 后，系统水平上 PVY 的积累增加。这些结果提供了关于 PCaP 在马铃薯 Y 病毒感染过程中作用的进一步知识，并揭示了其在不同病理系统中的作用差异。
