Departamento de Biologia Celular, Universidade de Brasília, Brasília, Brazil; Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil.
Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil.
J Proteomics. 2020 Apr 15;217:103690. doi: 10.1016/j.jprot.2020.103690. Epub 2020 Feb 14.
Arachis stenosperma is a wild peanut relative exclusive to South America that harbors high levels of resistance against several pathogens, including the peanut root-knot nematode (RKN) Meloidogyne arenaria. In this study, a proteomic survey of A. stenosperma-M. arenaria interaction using 2-DE and LC-MS/MS identified approximately 1400 proteins, out of which 222 were differentially abundant (DAPs) when RKN inoculated root samples were compared to the control. Most of these DAPs were assigned to functional categories related to plant responses to pathogens including stress, glycolysis, redox and tricarboxylic acid cycle. The comparison between the transcriptome (RNA-Seq) and proteome expression changes, showed that almost 55% of these DAPs encode genes with a similar expression trend to their protein counterparts. Most of these genes were induced during RKN infection and some were related to plant defense, such as MLP-like protein 34 (MLP34), cinnamoyl-CoA reductase 1 (CCR1), enolase (ENO), alcohol dehydrogenase (ADH) and eukaryotic translation initiation factor 5A (eIF5A). The overexpression of AsMLP34 in Agrobacterium rhizogenes transgenic roots in a susceptible peanut cultivar showed a reduction in the number of M. arenaria galls and egg masses, indicating that AsMLP34 is a promising candidate gene to be exploited in breeding programs for RKN control in peanut. SIGNIFICANCE: The use of an integrated approach to compare plant-nematode transcriptional and translational data enabled the identification of a new gene, AsMLP34, for Meloidogyne resistance.
硬实野花生是一种仅在南美洲存在的野生花生近缘种，对包括花生根结线虫（RKN）南方根结线虫（Meloidogyne arenaria）在内的多种病原体具有高水平的抗性。在这项研究中，通过 2-DE 和 LC-MS/MS 对 A. stenosperma-M. arenaria 互作进行了蛋白质组学调查，鉴定出了约 1400 种蛋白质，其中 222 种在 RKN 接种根样与对照相比时丰度差异显著（DAP）。这些 DAP 大多数被分配到与植物对病原体的反应相关的功能类别，包括应激、糖酵解、氧化还原和三羧酸循环。转录组（RNA-Seq）和蛋白质组表达变化的比较表明，这些 DAP 中的近 55%编码与蛋白对应物具有相似表达趋势的基因。这些基因大多数在 RKN 感染期间被诱导，有些与植物防御有关，如 MLP 样蛋白 34（MLP34）、肉桂酰辅酶 A 还原酶 1（CCR1）、烯醇酶（ENO）、醇脱氢酶（ADH）和真核翻译起始因子 5A（eIF5A）。在感病花生品种中，将 AsMLP34 过表达在发根农杆菌转基因根中，可减少南方根结线虫的根结和卵块数量，表明 AsMLP34 是一种很有前途的候选基因，可以用于花生根结线虫控制的育种计划。意义：使用整合的方法比较植物-线虫的转录和翻译数据，鉴定出一个新的基因 AsMLP34，用于南方根结线虫的抗性。
