Ovarian expression, polymorphism identification and association of gene with reproduction traits in goats.

The NAD-dependent protein deacetylase, Sirtuin3 (SIRT3), plays a role in fertility by preventing the activation of reactive oxygen species (ROS). A novel study was conducted on caprine , to study its ovarian expression, explore the sequence variability in exon 7 and analyze its association with prolificacy in two native goat breeds of Kerala, Malabari and Attappady Black. The mRNA isolated from ovaries of six Malabari and Attappady Black goats were subjected to quantitative PCR (qPCR) using and as reference genes. Genomic DNA was isolated from 185 goats (99 Malabari and 86 Attappady Black) and subjected to PCR-SSCP to identify polymorphism in exon 7 of and association with litter size was analyzed. The ovarian expression of caprine was significantly higher ( ≤ 0.01) in Malabari than low prolific Attappady Black. PCR-SSCP analysis revealed, exon 7 of was polymorphic with three genotypes namely, AA, AB and BB with a novel SNP, g.154C > T in the 3'UTR. A significant association ( ≤ 0.05) was noticed between the genotypes of and litter size. The results obtained from this study highlight the role of in reproduction and hence may be considered as a potential candidate gene for genetic improvement in goats.