Department of Pharmacology, Kasetsart University, Bangkok 10900, Thailand.
Department of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
J Food Prot. 2020 Mar 1;83(3):497-502. doi: 10.4315/0362-028X.JFP-19-316.
This study was conducted to investigate possible contamination by aflatoxins (AFs) and aflatoxigenic fungi in imported chia seeds consumed in Thailand. A survey was performed on 100 samples of imported chia seeds collected from supermarkets and health food stores in Bangkok from May 2017 to February 2018. Ten mold species belonging to Aspergillus and Penicillium were isolated, and Aspergillus flavus was the most prevalent aflatoxigenic fungi. Chia seed samples were cleaned with an immunoaffinity column and analyzed for AFs by high-performance liquid chromatography with fluorescence detection using precolumn derivatization. AFs were detected in 40% of total samples at concentrations of 0.4 to 10.99 ng/g. Among the positive samples, three were contaminated with total AFs at concentrations higher than the European Union regulatory limit (4 ng/g). The most commonly found AF found in chia seeds was AFB1.
本研究旨在调查在泰国进口奇亚籽中是否存在黄曲霉毒素（AFs）和产黄曲霉毒素真菌的污染。2017 年 5 月至 2018 年 2 月，对曼谷超市和保健食品店采集的 100 份进口奇亚籽进行了调查。从样品中分离出 10 种属于曲霉属和青霉属的霉菌，其中黄曲霉是最常见的产黄曲霉毒素真菌。奇亚籽样品用免疫亲和柱进行清洗，用高效液相色谱法进行前柱衍生荧光检测分析 AFs。在总样品的 40%中检测到 AFs，浓度为 0.4 至 10.99ng/g。在阳性样品中，有三个样品的总 AFs 浓度高于欧盟法规限量（4ng/g）。在奇亚籽中发现的最常见的 AF 是 AFB1。
