Ghodke Prerna Sanjay, Hegde Rashmi, Ansari Waqas, Muglikar Sangeeta, Dholkawala Alia S
Department of Periodontology and Oral Implantology, M. A. Rangoonwala College of Dental Sciences and Research Centre, Pune, India.
Abeda Inamdar Senior College of Arts, Science and Commerce, Azam Campus, Pune, India.
J Oral Implantol. 2020 Aug 1;46(4):381-388. doi: 10.1563/aaid-joi-D-19-00104.
Biological implant failures are primarily related to biofilm, which can lead to peri-mucositis and, further on, peri-implantitis. The 810-nm diode laser has an affinity for pigmented chromophores, so its use in the peri-implant sulcus has a significant bactericidal effect on the black-pigmented anaerobes such as Porphyromonas gingivalis. Therefore, it can be used to eliminate or reduce the bacterial count in the peri-implant sulcular fluid (PISF), thus increasing the life of the implants and reducing the chances of failure. The purpose of this study was to evaluate the efficacy of the 810-nm diode laser for the maintenance of dental implants and its use as a regular in-office tool for limiting the microbiological count in the PISF. Twenty patients undergoing implant treatment at the Department of Periodontology and Oral Implantology were randomly selected for the study. PISF samples were collected before and after the sulcus was lased with an 810-nm diode laser and sent for quantitative microbiological analysis using universal bacterial count, and the quantity of P gingivalis was evaluated using real-time polymerase chain reaction (PCR). The analysis revealed that after diode application, the median percentage drop in the microbial count was 76.67% and the median percentage drop in P gingivalis count was 99.28%. The use of an 810-nm diode laser resulted in the following outcomes: (1) drastic reduction in the total bacterial count around the implant and (2) significant reduction in the P gingivalis count, as evaluated by real-time PCR.
生物植入物失败主要与生物膜有关，生物膜可导致种植体周围黏膜炎，进而发展为种植体周围炎。810纳米二极管激光对色素发色团具有亲和力，因此其在种植体周围龈沟的应用对牙龈卟啉单胞菌等黑色色素厌氧菌具有显著的杀菌作用。因此，它可用于消除或减少种植体周围龈沟液（PISF）中的细菌数量，从而延长种植体寿命并降低失败几率。本研究的目的是评估810纳米二极管激光对牙种植体维护的疗效，以及将其作为一种常规的门诊工具用于限制PISF中微生物数量的效果。随机选择20名在牙周病与口腔种植科接受种植治疗的患者参与本研究。在使用810纳米二极管激光照射龈沟前后采集PISF样本，并送去进行通用细菌计数的定量微生物分析，同时使用实时聚合酶链反应（PCR）评估牙龈卟啉单胞菌的数量。分析显示，应用二极管激光后，微生物数量的中位数下降百分比为76.67%，牙龈卟啉单胞菌数量的中位数下降百分比为99.28%。使用810纳米二极管激光产生了以下结果：（1）种植体周围的总细菌数量大幅减少；（2）通过实时PCR评估，牙龈卟啉单胞菌数量显著减少。
