Division of Spine Surgery, The First Affiliated Hospital of Guangxi Medical University, No.6 Shuangyong Road, Nanning, 530021, China.
Department of Orthopaedic Surgery, The First Affiliated Hospital of Guangxi Medical University, No.6 Shuangyong Road, Nanning, 530021, China.
J Orthop Surg Res. 2020 Feb 18;15(1):54. doi: 10.1186/s13018-020-01588-8.
To investigate the association between interleukin-6 (IL-6) (rs1800795, rs1800796, rs1800797, rs13306435, rs2069849) and interleukin-10 (IL-10) (rs1800871, rs1800896) gene polymorphisms, expression levels, and lumbar disc disease (LDD).
We conducted a literature research on PubMed, Embase, Web of Science, Cochrane Library, and China National Knowledge Infrastructure (CNKI) until February 28, 2019. We included all case-control studies about the association between IL-6 and IL-10 gene polymorphisms and LDD. The odds ratio (OR) and 95% confidence interval (CI) were calculated to estimate the strength of association. Statistical analysis was conducted by Review Manager (RevMan) 5.3 software. Furthermore, immunohistochemistry (IHC) and RT-PCR were performed to evaluate IL-6 and IL-10 expressions in the normal and degenerated disc.
A total of 6 studies, involving 1456 cases and 1611 controls, were included in this meta-analysis. G alleles of rs1800795 and rs1800797 in the IL-6 gene were significantly associated with LDD (rs1800795: G vs. C, OR = 1.38, 95% CI = 1.16-1.64, P = 0.0002; rs1800797: G vs. A, OR = 1.35, 95% CI = 1.14-1.61, P = 0.0006). Begg's funnel plot and Egger's tests did not show any evidence of publication bias. IL-6 expression and IL-6 mRNA levels were significantly increased in the degenerated disc compared with those in the normal disc (IL-6 immunopositive cells, 73.68 ± 10.99% vs. 37.23 ± 6.42%, P < 0.001).
IL-6 gene polymorphisms (rs1800795 and rs1800797) were significantly associated with susceptibility to LDD. A high expression level of IL-6 may be an important risk factor for LDD.
为了研究白细胞介素 6（IL-6）（rs1800795、rs1800796、rs1800797、rs13306435、rs2069849）和白细胞介素 10（IL-10）（rs1800871、rs1800896）基因多态性、表达水平与腰椎间盘疾病（LDD）的关系。
我们对 PubMed、Embase、Web of Science、Cochrane 图书馆和中国知网（CNKI）进行了文献检索，检索时间截至 2019 年 2 月 28 日。我们纳入了所有关于 IL-6 和 IL-10 基因多态性与 LDD 相关性的病例对照研究。使用 odds ratio（OR）和 95%置信区间（CI）来评估关联强度。统计分析采用 Review Manager（RevMan）5.3 软件进行。此外，我们通过免疫组织化学（IHC）和 RT-PCR 评估了正常和退变椎间盘的 IL-6 和 IL-10 表达。
共有 6 项研究，包括 1456 例病例和 1611 例对照，纳入了本次荟萃分析。IL-6 基因 rs1800795 和 rs1800797 的 G 等位基因与 LDD 显著相关（rs1800795：G 与 C，OR=1.38，95%CI=1.16-1.64，P=0.0002；rs1800797：G 与 A，OR=1.35，95%CI=1.14-1.61，P=0.0006）。Begg 漏斗图和 Egger 检验均未显示出发表偏倚的证据。与正常椎间盘相比，退变椎间盘的 IL-6 表达和 IL-6 mRNA 水平显著升高（IL-6 免疫阳性细胞，73.68±10.99% vs. 37.23±6.42%，P<0.001）。
IL-6 基因多态性（rs1800795 和 rs1800797）与 LDD 的易感性显著相关。IL-6 高表达可能是 LDD 的一个重要危险因素。
