利用傅里叶变换红外光谱技术追踪活细胞中的糖基化。
Tracking glycosylation in live cells using FTIR spectroscopy.
机构信息
Institute of Pharmaceutical Science, King's College London, SE1 9NH, UK; Biological Science, College of Natural Sciences and Mathematics, California State University Fullerton, USA.
Institute of Pharmaceutical Science, King's College London, SE1 9NH, UK.
出版信息
Talanta. 2020 May 1;211:120737. doi: 10.1016/j.talanta.2020.120737. Epub 2020 Jan 13.
Abstract
This is the first demonstration of the study of glycan protein turnover in living cells by FTIR with commercially available tetraacetylated N-Azidoacetyl-D-Mannosamine (AcManNAz) label. The FTIR analysis has shown to be able to monitor the metabolism of glycans in living cells in real time. The method is simple, quantitative and requires equipment that are available in many laboratories. It can be used in a wide range of applications such as the study of glycosylation and cell-signalling.
摘要
这是首次通过傅里叶变换红外光谱（FTIR）利用市售的四乙酰基 N-叠氮乙酰基-D-甘露糖胺（AcManNAz）标记物研究活细胞中糖蛋白周转率的研究。FTIR 分析已被证明能够实时监测活细胞中聚糖的代谢。该方法简单、定量，所需设备在许多实验室都有。它可以应用于广泛的领域，如糖基化和细胞信号转导的研究。
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