脊髓抑制性神经受体在温水浸浴疗法的抗痛觉过敏效应中的作用。
The role of spinal inhibitory neuroreceptors in the antihyperalgesic effect of warm water immersion therapy.
机构信息
Experimental Neuroscience Laboratory (LaNEx), Universidade do Sul de Santa Catarina, Palhoça, SC, Brazil; Postgraduate Program in Health Sciences, Universidade do Sul de Santa Catarina, Palhoça, SC, Brazil.
Experimental Neuroscience Laboratory (LaNEx), Universidade do Sul de Santa Catarina, Palhoça, SC, Brazil; Laboratory of Bioenergetics and Oxidative Stress (LABOX), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.
出版信息
Braz J Phys Ther. 2021 Jan-Feb;25(1):56-61. doi: 10.1016/j.bjpt.2020.02.001. Epub 2020 Feb 12.
OBJECTIVE
Warm water immersion therapy (WWIT) has been widely used in the treatment of various clinical conditions, with analgesic and anti-inflammatory effects. However, its mechanism of action has not been fully investigated. The present study analyzed the role of spinal inhibitory neuroreceptors in the antihyperalgesic effect of WWIT in an experimental model of inflammatory pain.
METHODS
Mice were injected with complete Freund's adjuvant (CFA; intraplantar [i.pl.]). Paw withdrawal frequency to mechanical stimuli (von Frey test) was used to determine: (1) the effect of intrathecal (i.t.) preadministration of naloxone (a non-selective opioid receptor antagonist; 5 µg/5 µl), (2); AM281 (a selective cannabinoid receptor type 1 [CB] antagonist; 2 µg/5 µl), (3); and 1,3-dipropyl-8-cyclopentylxanthine (DPCPX; a selective adenosine A receptor antagonist; 10 nmol/5 µl), on the antihyperalgesic (pain-relieving) effect of WWIT against CFA-induced hyperalgesia.
RESULTS
Intrathecal naloxone, AM281, and DPCPX significantly prevented the antihyperalgesic effect of WWIT. This study suggests the involvement of spinal (central) receptors in the antihyperalgesic effect of WWIT in a model of inflammatory pain.
CONCLUSIONS
Taken together, these results suggest that opioid, CB and A spinal receptors might contribute to the pain-relieving effect of WWIT.
目的
温水浸浴疗法（WWIT）已广泛应用于各种临床病症的治疗，具有镇痛和抗炎作用。然而，其作用机制尚未得到充分研究。本研究分析了脊髓抑制性神经受体在 WWIT 治疗炎症性疼痛实验模型中的抗痛觉过敏作用。
方法
向小鼠注射完全弗氏佐剂（CFA；足底内[ip.]）。利用机械刺激（von Frey 测试）来测定：（1）鞘内（i.t.）预先给予纳洛酮（非选择性阿片受体拮抗剂；5 µg/5 µl）、（2）AM281（选择性大麻素受体 1 [CB]拮抗剂；2 µg/5 µl）和（3）1,3-二丙基-8-环戊基黄嘌呤（DPCPX；选择性腺苷 A 受体拮抗剂；10 nmol/5 µl）对 WWIT 对抗 CFA 诱导的痛觉过敏的抗痛觉过敏（镇痛）作用的影响。
结果
鞘内纳洛酮、AM281 和 DPCPX 显著阻止了 WWIT 的抗痛觉过敏作用。本研究表明，脊髓（中枢）受体参与了 WWIT 在炎症性疼痛模型中的抗痛觉过敏作用。
结论
综上所述，这些结果表明，阿片类、CB 和 A 脊髓受体可能有助于 WWIT 的镇痛作用。
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