Luna-Rodríguez Carolina E, Treviño-Rangel Rogelio de J, Soto-Domínguez Adolfo, Becerril-García Miguel A, González-Montalvo Martin A, Saldivar M Andrea M, Rodríguez-Rocha Humberto, Gonzalez Gloria M
Department of Microbiology, Universidad Autónoma de Nuevo León, Facultad de Medicina and Hospital Universitario "Dr. José Eleuterio González", Av. Francisco I. Madero, Mitras Centro, 64460, Monterrey, Mexico.
Department of Histology, Universidad Autónoma de Nuevo León, Facultad de Medicina and Hospital Universitario "Dr. José Eleuterio González", Av. Francisco I. Madero, Mitras Centro, 64460, Monterrey, Mexico.
Microb Pathog. 2020 May;142:104073. doi: 10.1016/j.micpath.2020.104073. Epub 2020 Feb 15.
A pulmonary infection model due to Scedosporium apiospermum in immunocompetent mice was developed. BALB/c mice were infected by endotracheal intubation with 5 × 10 conidia/mouse and disease progression was evaluated on days 1, 3, 5, 7, 11, 16, 21, 30, 50 and 60 post-infection through quantitative culture and histopathological analysis of lungs, livers, spleens, brains, and kidneys. There was no extrapulmonary dissemination during the study nor shown to be a lethal infection. The fungal burden in lungs was maintained from day 1-5 and gradually decreased by day 30 post-challenge. On day 60, 30% of mice showed complete elimination of the fungus. Severe alterations in the lung tissue were observed, as well as the presence of conidia and hyphae surrounded by a cellular infiltrate composed mainly of neutrophils in the first days of the infection. The elimination of fungal cells and normal tissue morphology were recovered throughout the study.
建立了免疫功能正常小鼠的由尖端赛多孢菌引起的肺部感染模型。通过气管插管给BALB/c小鼠接种5×10个分生孢子/只，在感染后第1、3、5、7、11、16、21、30、50和60天，通过对肺、肝、脾、脑和肾进行定量培养和组织病理学分析来评估疾病进展。在研究期间没有肺外播散，也未显示为致死性感染。肺部真菌负荷在第1至5天维持稳定，在攻击后第30天逐渐下降。在第60天，30%的小鼠显示真菌被完全清除。在感染的最初几天观察到肺组织有严重改变，以及存在被主要由中性粒细胞组成的细胞浸润包围的分生孢子和菌丝。在整个研究过程中，真菌细胞的清除和正常组织形态得以恢复。
