Reproductive Medical Centre, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuchang District, Wuhan, 430060, China.
Reproductive Medical Centre, Renmin Hospital of Wuhan University, 238 Jiefang Road, Wuchang District, Wuhan, 430060, China.
Mol Cell Endocrinol. 2020 Apr 15;506:110760. doi: 10.1016/j.mce.2020.110760. Epub 2020 Feb 15.
Long non-coding RNAs (lncRNAs) play a vital role in the progression of many human diseases. The aim of this study is to explore the relationship between lncRNA-ovarian cancer associated 1 (Lnc-OC1) and PCOS. In this study, we found that Lnc-OC1 was significantly higher in PCOS granulosa cells (GCs) compared to non-PCOS GCs. Lnc-OC1 knockdown inhibited cell viability and promoted cell apoptosis, expression of aromatase mRNA and production of estradiol in KGN cells. In PCOS mice, Lnc-OC1 promoted the serum insulin release, production of angiogenesis-related factors and IκBα phosphorylation, which could be partially restored by Lnc-OC1 shRNA. These results suggest that Lnc-OC1 plays an important part in the pathogenesis of PCOS.
长链非编码 RNA（lncRNAs）在许多人类疾病的进展中起着至关重要的作用。本研究旨在探讨 lncRNA-卵巢癌相关 1（Lnc-OC1）与 PCOS 之间的关系。在这项研究中，我们发现 PCOS 颗粒细胞（GCs）中的 Lnc-OC1 明显高于非 PCOS GCs。Lnc-OC1 敲低抑制了 KGN 细胞的活力并促进了细胞凋亡、芳香化酶 mRNA 的表达和雌二醇的产生。在 PCOS 小鼠中，Lnc-OC1 促进了血清胰岛素的释放、血管生成相关因子的产生和 IκBα 的磷酸化，而这些作用可以部分被 Lnc-OC1 shRNA 所恢复。这些结果表明 Lnc-OC1 在 PCOS 的发病机制中起着重要作用。
