Cocoa beans of different origins and varieties and their derived products contamination with polycyclic aromatic hydrocarbons.

Levels of polycyclic aromatic hydrocarbons (PAHs) in cocoa beans of several varieties originating from different countries and their derived products from one technological line were examined. PAHs analysis was performed using HPLC-FLD/DAD and confirmed by GC-MS. Significant differences in total 19 PAHs contents between raw cocoa beans of different varieties and origins were observed. The highest sums of 19 PAHs were determined in roasted cocoa beans, cocoa mass and cocoa butter (16.69-74.15 μg kg of fat). The roasting temperature of 160 °C led to PAHs formation, though not the heavy ones. Lowering temperature to 140 °C while extending the time minimized the total contamination but to a small extent. In all samples relatively low levels of total contamination were noted, with light PAHs being predominant and the sum of 4 heavy and marker PAHs much lower than the maximum legal limit. Therefore, analysed products, especially chocolate, do not threaten consumers' health.