[大鼠咬合干扰致口面部疼痛敏感性的口面部操作性测试特征]
[Characteristics of orofacial operant test for orofacial pain sensitivity caused by occlusal interference in rats].
作者信息
Bai S S, Mo S Y, Xu X X, Liu Y, Xie Q F, Cao Y
机构信息
Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
Center for Oral and Jaw Functional Diagnosis, Peking University School and Hospital of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
出版信息
Beijing Da Xue Xue Bao Yi Xue Ban. 2020 Feb 18;52(1):51-57. doi: 10.19723/j.issn.1671-167X.2020.01.008.
OBJECTIVE
To compare the orofacial pain sensitivity with operant test and mechanical hyperalgesia with von Frey filaments of two orofacial pain models (EOI: experimental occlusal interference; pIONX: partial infraorbital nerve transection). To investigate the operant and evoked characteristics of EOI-rats.
METHODS
The orofacial operant behaviors were tested by Ugo Basile Orofacial Stimulation Test System. The mechanical thresholds of vibrissal pads were tested by von Frey filaments. Male Sprague-Dawley rats were randomly divided into eight groups: von Frey group: sham-EOI, EOI, sham-pIONX, pIONX (sham: sham-operated group); operant test group: sham-EOI, EOI, sham-pIONX, pIONX (sham: sham-operated group). The mechanical thresholds and orofacial operant behaviors were tested on pre-operation and post-operation days l, 3, 7, 10, 14 and 21.
RESULTS
In pIONX of von Frey group, the mechanical withdrawal threshold decreased from days 1 to 21 (P<0.05), peaking from days 7 to 10, and lasted until the end of the experiment. There was no significant difference between the bilateral sides. In pIONX of operant test group, the total contact time decreased from days 10 to 21 (P<0.05), peaking from days 10 to 14, and lasted until the end of the experiment. In EOI of von Frey group, the mechanical withdrawal threshold decreased from days 3 to 21 (P<0.05), peaking on day 7, and lasted until the end of the experiment. There was no significant difference between the bilateral sides. In EOI of operant test group, the total contact time decreased from days 1 to 21 (P<0.05), peaking from days 7 to 10, and lasting until the end of experiment.
CONCLUSION
Orofacial operant test is a stable method to evaluate orofacial pain behaviors, which could discriminate the feature of neuropathic and EOI orofacial pain. In these two animal models, both of the operant behaviors and the mechanical hyperalgesia exhibited different time courses. Orofacial operant test provides a novel method for evaluating the orofacial pain sensitivity and studying the orofacial pain mechanism thoroughly.
目的
比较两种口面部疼痛模型（EOI：实验性咬合干扰；pIONX：眶下神经部分切断术）通过操作性测试得出的口面部疼痛敏感性以及通过von Frey细丝得出的机械性痛觉过敏情况。研究EOI大鼠的操作性和诱发性特征。
方法
采用Ugo Basile口面部刺激测试系统对口面部操作性行为进行测试。用von Frey细丝测试触须垫的机械阈值。将雄性Sprague-Dawley大鼠随机分为八组：von Frey组：假手术-EOI、EOI、假手术-pIONX、pIONX（假手术：假手术组）；操作性测试组：假手术-EOI、EOI、假手术-pIONX、pIONX（假手术：假手术组）。在术前以及术后第1、3、7、10、14和21天测试机械阈值和口面部操作性行为。
结果
在von Frey组的pIONX中，机械性撤针阈值从第1天到第21天降低（P<0.05），在第7天到第10天达到峰值，并持续到实验结束。双侧之间无显著差异。在操作性测试组的pIONX中，总接触时间从第10天到第21天减少（P<0.05），在第10天到第14天达到峰值，并持续到实验结束。在von Frey组的EOI中，机械性撤针阈值从第3天到第21天降低（P<0.05），在第7天达到峰值，并持续到实验结束。双侧之间无显著差异。在操作性测试组的EOI中，总接触时间从第1天到第21天减少（P<0.05），在第7天到第10天达到峰值，并持续到实验结束。
结论
口面部操作性测试是评估口面部疼痛行为的一种稳定方法，可区分神经性和EOI口面部疼痛的特征。在这两种动物模型中，操作性行为和机械性痛觉过敏均表现出不同的时间进程。口面部操作性测试为评估口面部疼痛敏感性和深入研究口面部疼痛机制提供了一种新方法。
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