Verawatnapakul V, Pholpramool C
Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Andrologia. 1988 Sep-Oct;20(5):389-95.
Total, free and bound sialic acid concentrations were determined in sperm-free luminal fluid removed by micropuncture from different regions of the rat and hamster epididymides. In the rat, total sialic acid concentrations (mean +/- s.e.m.) in the proximal caput, the mid corpus and the proximal cauda were, respectively, 25.7 +/- 1.3, 23.9 +/- 1.7 and 28.8 +/- 1.9 mM compared to 4.4 +/- 0.1 mM in blood plasma. In the hamster, total sialic acid concentrations in the distal corpus, the proximal cauda and the distal cauda were, respectively, 32.9 +/- 3.8, 26.4 +/- 1.4 and 26.6 +/- 3.2 mM compared to 4.7 +/- 0.5 mM in blood plasma. Free sialic acid accounted for approximately 70-80% of total sialic acid present in the epididymal plasma of the rat. Similarly, 82% of sialic acid in the rat blood plasma was in free form. The levels of free and bound sialic acid were not changed in different regions of the rat epididymis. Unilateral ligation of the rat efferent ducts had no effect on total, free or bound sialic acid concentrations in all regions except in the proximal cauda in which a transient increase (P less than 0.01) in free sialic acid was observed on Day-3 after EDL.
通过微穿刺法从大鼠和仓鼠附睾不同区域采集无精子的管腔液，测定其中总唾液酸、游离唾液酸和结合唾液酸的浓度。在大鼠中，附睾头近端、附睾体中部和附睾尾近端的总唾液酸浓度（均值±标准误）分别为25.7±1.3、23.9±1.7和28.8±1.9 mM，而血浆中为4.4±0.1 mM。在仓鼠中，附睾体远端、附睾尾近端和附睾尾远端的总唾液酸浓度分别为32.9±3.8、26.4±1.4和26.6±3.2 mM，血浆中为4.7±0.5 mM。游离唾液酸约占大鼠附睾血浆中总唾液酸的70 - 80%。同样，大鼠血浆中82%的唾液酸为游离形式。大鼠附睾不同区域的游离和结合唾液酸水平没有变化。单侧结扎大鼠输出小管对所有区域的总唾液酸、游离唾液酸或结合唾液酸浓度均无影响，但在结扎后第3天，附睾尾近端的游离唾液酸出现短暂升高（P＜0.01）。
