关于短线性DNA模板和磷酸胍引物在使用Bst外切酶DNA聚合酶进行等温扩增过程中的多聚化效率的数据。
Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase.
作者信息
Garafutdinov Ravil R, Sakhabutdinova Assol R, Kupryushkin Maxim S, Pyshnyi Dmitrii V
机构信息
Institute of Biochemistry and Genetics, Ufa Federal Research Center of the Russian Academy of Sciences, Russian Federation.
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Russian Federation.
出版信息
Data Brief. 2020 Jan 25;29:105188. doi: 10.1016/j.dib.2020.105188. eCollection 2020 Apr.
This article reports experimental data related to the research article entitled "Prevention of DNA multimerization using phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase" (R.R. Garafutdinov, A.R. Sakhabutdinova, M.S. Kupryushkin, D.V. Pyshnyi, 2020) [1]. Here, multimerization efficiency in terms of Tt (time-to-threshold) values obtained for artificial DNA templates with the different nucleotide sequences during isothermal amplification with Bst exo- DNA polymerase is given. Data on the influence of phosphoryl guanidine primers (PGO) on multimerization for the LTc template which has shown high efficiency of multimerization are presented as well.
本文报道了与题为《使用磷酸胍引物在Bst exo- DNA聚合酶等温扩增过程中防止DNA多聚体形成》（R.R. 加拉富季诺夫、A.R. 萨哈布季诺娃、M.S. 库普留什金、D.V. 皮什尼，2020年）[1]的研究论文相关的实验数据。此处给出了在使用Bst exo- DNA聚合酶进行等温扩增期间，针对具有不同核苷酸序列的人工DNA模板获得的以Tt（达到阈值时间）值表示的多聚体形成效率。还展示了磷酸胍引物（PGO）对已显示出高效多聚体形成的LTc模板多聚体形成影响的数据。
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