Feodorova Valentina A, Zaitsev Sergey S, Khizhnyakova Mariya A, Saltykov Yury V, Evstifeev Vitaliy V, Khusainov Fidail M, Yakovlev Sergey I, Larionova Olga S, Motin Vladimir L
Laboratory for Molecular Biology and NanoBiotechnology, Federal Research Center for Virology and Microbiology (FRCViM), Branch in Saratov, 410028, Saratov, Russia.
Laboratory of Viral and Chlamydial Infections, Federal Center for Toxicological, Radiation and Biological Safety, 420074, Kazan, Republic of Tatarstan, Russia.
Data Brief. 2020 Jan 27;29:105190. doi: 10.1016/j.dib.2020.105190. eCollection 2020 Apr.
are obligate intracellular bacteria globally widespread across humans, wildlife, and domesticated animals. is a primarily zoonotic pathogen with multiple hosts, which can be transmitted to humans, resulting in psittacosis or ornithosis. Since this pathogen is a well-recognized threat to human and animal health, it is critical to unravel in detail the genetic make-up of this microorganism. Though many genomes of have been studied to date, little is known about the variants of chlamydial organisms causing infection in Russian livestock. This research is the first genome assembly of the strain Rostinovo-70 of zoonotic origin that was isolated in Russian Federation. The results were obtained by using standard protocols of sequencing with the Illumina HiSeq 2500 and Oxford Nanopore MinION technology that generated 3.88 GB and 3.08 GB of raw data, respectively. The data obtained are available in NCBI DataBase (GenBank accession numbers are CP041038.1 & CP041039.1). The Multi-Locus Sequence Typing (MLST) showed that the strain Rostinovo-70 together with GR9 and C WS/RT/E30 belong to the sequence type (ST)28 that could be further separated into two different clades. Despite Rostinovo-70 and GR9 formed a single clade, the latter strain did not contain a cryptic plasmid characteristis to Rostinovo-70. Moreover, the genomes of two strains differed significantly in the cluster of 30 genes that in Rostinovo-70 were closer to rather than . The alignment of the genomes of and in this area revealed the exact boarders of homologous recombination that occurred between two Chlamydia species. These findings provide evidence for the first time of genetic exchange between closely related Chlamydia species.
是专性胞内细菌，在全球范围内广泛存在于人类、野生动物和家畜中。是一种主要的人畜共患病原体，有多个宿主，可传播给人类，导致鹦鹉热或鸟疫。由于这种病原体是对人类和动物健康的公认威胁，详细解析这种微生物的基因组成至关重要。尽管迄今为止已经研究了许多的基因组，但对于在俄罗斯家畜中引起感染的衣原体生物变体知之甚少。本研究是首次对源自俄罗斯联邦的人畜共患病原体罗斯蒂诺沃 - 70菌株进行基因组组装。结果是通过使用Illumina HiSeq 2500测序标准方案和牛津纳米孔MinION技术获得的，分别产生了3.88 GB和3.08 GB的原始数据。所获得的数据可在NCBI数据库中获取（GenBank登录号为CP041038.1和CP041039.1）。多位点序列分型（MLST）表明，罗斯蒂诺沃 - 70菌株与GR9和C WS/RT/E30属于序列类型（ST）28，可进一步分为两个不同的进化枝。尽管罗斯蒂诺沃 - 70和GR9形成了一个单一的进化枝，但后者菌株不包含罗斯蒂诺沃 - 70特有的隐蔽质粒。此外，两种菌株的基因组在30个基因簇上有显著差异，在罗斯蒂诺沃 - 70中这些基因更接近而不是。在该区域和的基因组比对揭示了两个衣原体物种之间发生同源重组的确切边界。这些发现首次为密切相关的衣原体物种之间的基因交换提供了证据。
