大鼠圆形精子细胞的代谢：棉酚对葡萄糖转运的影响。

Metabolism of round spermatids in the rat: effect of gossypol on the glucose transport.

作者信息

Nakamura M, Ikeda M, Okinaga S, Arai K

机构信息

Department of Obstetrics and Gynecology, School of Medicine, Teikyo University, Tokyo/Japan.

出版信息

Andrologia. 1988 Sep-Oct;20(5):411-6. doi: 10.1111/j.1439-0272.1988.tb00714.x.

DOI:10.1111/j.1439-0272.1988.tb00714.x

PMID:3207199

Abstract

Low dose of gossypol (5 microM) completely inhibited uptake of 2-deoxy-D-glucose by rat spermatids. With gossypol 2-deoxy-D-glucose transport into spermatids increased curvelinearly for 10 minutes after the start of incubation, but reached a plateau afterwards, whereas without gossypol it continuously increased. The levels of fructose-1,6-bis-phosphate (FBP) and ATP were inversely correlated when the cells were exposed to glucose. However, in the presence of gossypol the levels of ATP and FBP decreased simultaneously. In addition, gossypol at 10 microM stimulated oxygen consumption when lactate was present. These results suggest that gossypol probably inhibits glucose transport into spermatids by lowering the ATP level by uncoupling respiratory chain-linked phosphorylation.

摘要

低剂量棉酚（5微摩尔）完全抑制大鼠精子细胞对2-脱氧-D-葡萄糖的摄取。在有棉酚的情况下，孵育开始后10分钟内，2-脱氧-D-葡萄糖向精子细胞的转运呈曲线增加，但随后达到平台期，而在没有棉酚的情况下，其转运持续增加。当细胞暴露于葡萄糖时，果糖-1,6-二磷酸（FBP）和ATP的水平呈负相关。然而，在有棉酚存在时，ATP和FBP的水平同时下降。此外，当存在乳酸时，10微摩尔的棉酚刺激氧气消耗。这些结果表明，棉酚可能通过解偶联呼吸链相关的磷酸化作用降低ATP水平，从而抑制葡萄糖向精子细胞的转运。