基因编辑技术CRISPR/Cas在斑马鱼中的研究进展与应用
[Research progress and applications of gene editing technology CRISPR/Cas in zebrafish].
作者信息
Ouyang Juan, Xue Songlei, Zhou Qiqi, Cui Hengmi
机构信息
Institute of Epigenetics & Epigenomics, Yangzhou University, Yangzhou 225009, Jiangsu, China.
出版信息
Sheng Wu Gong Cheng Xue Bao. 2020 Jan 25;36(1):1-12. doi: 10.13345/j.cjb.190178.
Clustered regularly interspaced short palindromic repeats (CRISPR) are acquired immune system in bacteria and archaea. This system is used in site-directed gene editing. Recently, scientists discovered new CRISPR-associated (Cas) proteins, in which Cas12a-mediated gene editing can significantly reduce the off-target rate. In this article, we review CRISPR/Cas system's discovery of history, composition, classification, and working principle. The latest research progress of the CRISPR/Cas system, and its application in zebrafish are introduced.
成簇规律间隔短回文重复序列（CRISPR）是细菌和古细菌中的获得性免疫系统。该系统用于定点基因编辑。最近，科学家发现了新的CRISPR相关（Cas）蛋白，其中Cas12a介导的基因编辑可显著降低脱靶率。在本文中，我们综述了CRISPR/Cas系统的发现历史、组成、分类和工作原理。介绍了CRISPR/Cas系统的最新研究进展及其在斑马鱼中的应用。
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