Silveira Simone, Cibulski Samuel Paulo, Junqueira Dennis Maletich, Mósena Ana Cristina Sbaraini, Weber Matheus Nunes, Mayer Fabiana Quoos, Canal Cláudio Wageck
Laboratório de Virologia, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil.
Faculdade de Medicina Veterinária, Universidade do Oeste de Santa Catarina (UNOESC), Xanxerê, Brazil.
Transbound Emerg Dis. 2020 Sep;67(5):1909-1917. doi: 10.1111/tbed.13520. Epub 2020 Mar 4.
The HoBi-like pestivirus (HoBiPeV), currently classified as Pestivirus H species, is a pathogen associated with a broad spectrum of clinical manifestations in ruminants, particularly in cattle. Since HoBiPeV complete genome sequencing data is scarce, in the present study we described five nearly complete new Brazilian HoBiPeV genomes and further perform a more complete genetic and evolutionary characterization with all additional genome sequences available in the GenBank database. Entropy and selection pressure analysis showed the E2 gene, a surface glycoprotein, is the most variable gene, which also displays the greatest number of sites under positive selection. Phylogenetic and Bayesian inference based on complete genome and N gene, respectively, from all HoBiPeV sequences available so far, confirms the existence of three main clades (a, b, and c). The abovementioned analysis suggests that this pestivirus species probably emerged in Asia and spread to different regions including Brazil, where only strains belonging to specific genetic group 'a' have been found. The hypothesis of the HoBiPeV introduction in Brazil (between 1,890 and 1,962), formulated based on Bayesian inference, coincides with a period of intensive importation of water buffalo (Bubalus arnee) and indicine cattle (Bos taurus indicus) from Asia to Brazil, suggesting that this could be the origin of the current Brazilian HoBiPeV genetic group 'a'.
类霍比瘟病毒（HoBiPeV），目前被归类为瘟病毒属H种，是一种与反刍动物，尤其是牛的广泛临床表现相关的病原体。由于HoBiPeV完整基因组测序数据稀缺，在本研究中，我们描述了五个新的近乎完整的巴西HoBiPeV基因组，并利用GenBank数据库中所有可用的其他基因组序列进行了更全面的遗传和进化特征分析。熵和选择压力分析表明，表面糖蛋白E2基因是变异最大的基因，同时也显示出最多的正选择位点。分别基于所有现有HoBiPeV序列的完整基因组和N基因进行的系统发育分析和贝叶斯推断，证实了三个主要进化枝（a、b和c）的存在。上述分析表明，这种瘟病毒可能起源于亚洲，并传播到包括巴西在内的不同地区，在巴西仅发现了属于特定遗传组“a”的毒株。基于贝叶斯推断得出的HoBiPeV于1890年至1962年间传入巴西的假设，与亚洲水牛（Bubalus arnee）和印度牛（Bos taurus indicus）大量进口到巴西的时期相吻合，这表明这可能是当前巴西HoBiPeV遗传组“a”的起源。
