Laboratório de Fisiologia Animal, Universidade Federal do Amazonas - UFAM, Av. General Rodrigo Octávio Jordão Ramos, 3000, CEP 69077-000, Manaus, AM, Brasil.
Programa de Pós-graduação em Recursos Pesqueiros e Engenharia de Pesca - PREP, Universidade Estadual do Oeste do Paraná - UNIOESTE, Rua da Faculdade, 645, Jardim La Salle, CEP 85903-000, Toledo, PR, Brasil.
Braz J Biol. 2021 Jan-Feb;81(1):62-68. doi: 10.1590/1519-6984.219409.
The objective of this study was to characterize and compare the hematological variables (erythrogram, thrombogram, leukogram and plasma metabolites) of three cichlid species: Cichla monoculus, Cichla temensis and Cichla vazzoleri. A total of 45 specimens were captured in Balbina lake, Presidente Figueiredo, Amazonas, Brazil, with the aid of a rod and reel or hand line, with natural or artificial bait: 15 C. monoculus, 15 C. temensis and 15 C. vazzoleri. Their blood was removed by means of caudal puncture of the dorsal aorta, and hematological data were determined in accordance with methodology previously described in the literature. The erythrogram showed similarities between the species, while the thrombogram showed differences between C. vazzoleri and the other species studied (C. monoculus and C. temensis). The total leukocyte counts for C. temensis and C. vazzoleri were higher than those of C. monoculus. The predominant leukocyte in C. temensis and C. vazzoleri was lymphocytes, whereas it was monocytes in C. monoculus. The plasma metabolites showed differences between the three cichlid species, regarding their glucose, cholesterol, urea and potassium levels. It is concluded that these three species present hematological differentiation, thus indicating that they have differentiated blood-cell immune responses and plasma metabolite physiology.
本研究旨在对三种慈鲷鱼类（Cichla monoculus、Cichla temensis 和 Cichla vazzoleri）的血液学变量（红细胞图谱、血小板图谱、白细胞图谱和血浆代谢物）进行特征描述和比较。在巴西亚马逊州总统菲格雷多的巴尔比纳湖，我们使用鱼竿和卷线器或手线捕获了 45 个样本，诱饵为天然或人工：15 个 C. monoculus、15 个 C. temensis 和 15 个 C. vazzoleri。通过尾动脉的背侧穿刺采集血液，并按照文献中先前描述的方法确定血液学数据。红细胞图谱显示这些物种之间存在相似性，而血小板图谱显示 C. vazzoleri 与其他研究的物种（C. monoculus 和 C. temensis）之间存在差异。C. temensis 和 C. vazzoleri 的总白细胞计数高于 C. monoculus。C. temensis 和 C. vazzoleri 中的主要白细胞是淋巴细胞，而 C. monoculus 中的主要白细胞是单核细胞。三种慈鲷鱼类的血浆代谢物在葡萄糖、胆固醇、尿素和钾水平方面存在差异。结论是，这三个物种具有血液学分化，表明它们具有分化的血细胞免疫反应和血浆代谢生理学。
