Institute of Evolutionary Biology (IBE), CSIC-Universitat Pompeu Fabra, Barcelona, Spain.
Philos Trans R Soc Lond B Biol Sci. 2020 Mar 30;375(1795):20190341. doi: 10.1098/rstb.2019.0341. Epub 2020 Feb 10.
Most of the genotype-phenotype analyses to date have largely centred attention on single nucleotide polymorphisms. However, transposable element (TE) insertions have arisen as a plausible addition to the study of the genotypic-phenotypic link because of to their role in genome function and evolution. In this work, we investigate the contribution of TE insertions to the regulation of gene expression in response to insecticides. We exposed four strains to malathion, a commonly used organophosphate insecticide. By combining information from different approaches, including RNA-seq and ATAC-seq, we found that TEs can contribute to the regulation of gene expression under insecticide exposure by rewiring -regulatory networks. This article is part of a discussion meeting issue 'Crossroads between transposons and gene regulation'.
迄今为止，大多数的基因型-表型分析主要集中在单核苷酸多态性上。然而，转座元件（TE）的插入已经成为研究基因型-表型联系的一个合理补充，因为它们在基因组功能和进化中发挥作用。在这项工作中，我们研究了 TE 插入在调节基因表达以应对杀虫剂方面的作用。我们让四个品系接触马拉硫磷，一种常用的有机磷杀虫剂。通过结合 RNA-seq 和 ATAC-seq 等不同方法的信息，我们发现 TE 可以通过重新布线调控网络来促进杀虫剂暴露下的基因表达调控。本文是“转座子与基因调控的交叉点”讨论专题的一部分。
