Department of Laboratory Medicine, Ningbo First Hospital, Ningbo, China.
Department of Laboratory Medicine, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, China.
J Antibiot (Tokyo). 2020 May;73(5):314-319. doi: 10.1038/s41429-020-0284-7. Epub 2020 Feb 19.
Multidrug-resistant (MDR) uropathogenic Escherichia coli (UPEC) are prevalent throughout the world resulting in a major public health burden. In this research, we isolated and identified 28 MDR UPEC from one university hospital in China, investigated MDR and pathogenic mechanisms by PCR, including 55 antibiotic resistance determinants (ARDs) genes, 13 genetic markers of mobile genetic elements (MGEs) and 6 virulence factors (VFs) genes. In these isolates, we identified 23 ARDs genes and 6 genetic markers of MGEs that played a key role in MDR phenotypes. In addition, we found 2 VFs genes, hofQ and ompT, which could be associated with pathogenicity and invasiveness of these strains in urinary tract infections (UTIs).
多药耐药（MDR）尿路致病性大肠杆菌（UPEC）在全球广泛流行，给公共卫生带来了重大负担。在这项研究中，我们从中国的一家大学医院分离并鉴定了 28 株 MDR UPEC，通过 PCR 研究了 MDR 和发病机制，包括 55 个抗生素耐药决定因子（ARDs）基因、13 个移动遗传元件（MGEs）的遗传标记和 6 个毒力因子（VF）基因。在这些分离株中，我们鉴定出了 23 个 ARD 基因和 6 个 MGE 的遗传标记，它们在 MDR 表型中起关键作用。此外，我们还发现了 2 个 VF 基因，hofQ 和 ompT，它们可能与这些菌株在尿路感染（UTIs）中的致病性和侵袭性有关。
