中国甘肃省牛巴贝斯虫和泰勒虫属的分子调查和遗传多样性。
Molecular Survey and Genetic Diversity of Babesia spp. and Theileria spp. in Cattle in Gansu Province, China.
机构信息
State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, 730046, Gansu, People's Republic of China.
Animal Husbandry and Veterinary Station of Minqin County, Wuwei, 733000, Gansu, People's Republic of China.
出版信息
Acta Parasitol. 2020 Jun;65(2):422-429. doi: 10.2478/s11686-020-00179-1. Epub 2020 Feb 19.
PURPOSE
Babesia spp. and Theileria spp. are tick-borne pathogens of livestock globally. In this study, we investigated the presence and distribution of these pathogens in cattle from 20 locations in 4 Counties of Wuwei City. The aim of the present research was to evaluate the spread of piroplasms, so as to provide the epidemiological information for control piroplasmosis in the region.
METHODS
The authors provided the molecular data for Babesia spp. and Theileria spp. and analyzed the obtained sequences of the 18S rRNA gene, Tams1 gene and MPSP gene by using the ClustalW program in MEGA version 6.06 software and BLASTn tool of NCBI GenBank database.
RESULTS
The total infection rates were detected by nPCR with 1.8% for T. orientalis, 3% for T. sinensis, 0.6% for T. annulata, 1.8% for B. motasi and 0.6% for B. bigemina.
CONCLUSIONS
To the best of our knowledge, this is the first report investigating T. sinensis from cattle by PCR in Wuwei City. In particular, ovine B. motasi has been for the first time detected in cattle in our study and its impact is worth discussing to figure out the potential reasons.
目的
巴贝西虫属和泰勒虫属是全球牲畜的蜱传病原体。本研究调查了武威市 4 个县 20 个地点牛群中这些病原体的存在和分布情况。本研究的目的是评估梨形虫的传播情况，为该地区控制梨形虫病提供流行病学信息。
方法
作者提供了巴贝西虫属和泰勒虫属的分子数据，并使用 MEGA 版本 6.06 软件的 ClustalW 程序和 NCBI GenBank 数据库的 BLASTn 工具对获得的 18S rRNA 基因、Tams1 基因和 MPSP 基因序列进行了分析。
结果
通过 nPCR 检测到总感染率为 1.8%的东方泰勒虫、3%的中华泰勒虫、0.6%的环形泰勒虫、1.8%的莫氏巴贝斯虫和 0.6%的双芽巴贝斯虫。
结论
据我们所知，这是首次在武威市通过 PCR 检测牛的中华泰勒虫。特别是，我们首次在牛中检测到绵羊莫氏巴贝斯虫，其影响值得讨论，以找出潜在的原因。
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