Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing210095, People's Republic of China.
Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Nanjing Agricultural University, Nanjing210095, People's Republic of China.
Br J Nutr. 2020 Jun 14;123(11):1258-1268. doi: 10.1017/S000711452000063X. Epub 2020 Feb 20.
The objective of this study was to explore the metabolic profiles of pregnancy malnutrition induced by feed restriction (FR) and the counteracting effects of glycerol and rumen-protected choline chloride supplementation. Two feeding trials were conducted. In the first experiment, twenty pregnant Hu sheep carrying multiple fetuses with a gestation period of 108 d were randomly divided into two groups. The ewes in the control (CON) group were offered 100 % of their nutritional requirements as recommended by the National Research Council (NRC), while the FR group was offered 30 % of feed intake of CON for 15 d. In the second experiment, eighteen pregnant Hu sheep were offered a feed intake comprising 30 % of the NRC-recommended nutritional requirements twice daily. The sheep were randomly divided into three groups: the FR group in the second experiment (FR2), with no supplementation, the glycerol (GLY) group, which received 40 ml of glycerol per d, and the rumen-protected choline chloride (RPC) group, which received 10 g of rumen-protected choline chloride per d for 9 d. In the first experiment, the urine metabolome of sixteen ewes showed significant difference between the CON group and FR group. Compared with the CON group, FR decreased the level of d-glucose, lactic acid, levoglucosan, α-ketoglutarate, phosphohydroxypyruvic acid, glucose 6-phosphate and the methyl donors, while increasing the level of pyruvate, fumaric acid and carnitines in urine. Both the GLY and RPC treatments counteracted some of these changes and modulated the urine metabolome in advanced pregnant ewes suffering from malnutrition.
本研究旨在探讨限饲（FR）诱导的妊娠营养不良的代谢特征，以及甘油和包膜氯化胆碱对其的拮抗作用。进行了两项饲养试验。在第一个实验中，将 20 只携带多胎妊娠 108 天的湖羊随机分为两组。对照组（CON）的母羊提供 100%的营养需求，按美国国家研究委员会（NRC）推荐的标准；而 FR 组提供 CON 组 30%的饲料摄入量，持续 15 天。在第二个实验中，18 只怀孕的湖羊每日分两次提供相当于 NRC 推荐营养需求 30%的饲料摄入量。这些羊被随机分为三组：第二个实验的 FR 组（FR2），未添加任何物质；甘油（GLY）组，每天接受 40ml 甘油；包膜氯化胆碱（RPC）组，每天接受 10g 包膜氯化胆碱，共 9 天。在第一个实验中，16 只母羊的尿液代谢组在 CON 组和 FR 组之间表现出显著差异。与 CON 组相比，FR 降低了 d-葡萄糖、乳酸、左旋葡聚糖、α-酮戊二酸、磷酸羟丙酮酸、葡萄糖 6-磷酸和甲基供体的水平，同时增加了丙酮酸、富马酸和肉碱的水平。GLY 和 RPC 处理均能部分拮抗这些变化，并调节因营养不良而处于妊娠晚期的母羊的尿液代谢组。
