Zhang Hao, Zhao Fangfang, Nie Haitao, Ma Tiewei, Wang Ziyu, Wang Feng, Loor Juan J
Laboratory of Metabolic Manipulation of Herbivorous Animal Nutrition, College of Animal Science and Technology, Yangzhou University, #48, Weihui Road,Yangzhou 225009, P.R. China.
Jiangsu Engineering Technology Research Center of Mutton Sheep and Goat Industry, Nanjing Agricultural University, #1, Tongwei Road, Nanjing 210095, P.R. China.
Reprod Fertil Dev. 2018 Oct;30(11):1522-1531. doi: 10.1071/RD18047.
The aims of the present study were to determine whether dietary supplementation with N-carbamylglutamate (NCG) and rumen-protected l-arginine (RP-Arg) to underfed Hu sheep would improve fetal thymus development and immune function. From Day 35 to Day 110 of gestation, 32 Hu ewes carrying twin fetuses were randomly allocated to one of four groups (n=8 per group): 100% National Research Council (NRC)-recommended nutrient requirements (CON), 50% NRC recommendations (RES), 50% NRC recommendations supplemented with 20gday-1 RP-Arg (RES+ARG), and 50% NRC recommendations supplemented with 5gday-1 NCG (RES+NCG). Medullary thickness was increased (P<0.05) in RES compared with CON ewes, but was reduced (P<0.05) in both RES+ARG and RES+NCG ewes compared with RES ewes. There were no differences in superoxide dismutase and glutathione peroxidase activity or malondialdehyde levels in the RES+ARG and RES+NCG groups compared with the CON group (P>0.05). Concentrations of IgA, interleukin (IL)-1β and IL-10 in fetal umbilical cord blood were reduced (P<0.05) in RES compared with CON ewes, but were increased (P<0.05) in both RES+ARG and RES+NCG ewes. Expression of Bax, Fas and p53 mRNA was increased (P<0.05) in RES compared with CON ewes, but were reduced (P>0.05) in both RES+ARG and RES+NCG ewes. These results indicate that dietary supplementation with NCG and RP-Arg could help alleviate the negative effects of intrauterine growth restriction on fetal thymus development and immune function.
本研究的目的是确定给营养不足的湖羊日粮中添加N-氨甲酰谷氨酸（NCG）和瘤胃保护性L-精氨酸（RP-Arg）是否会改善胎儿胸腺发育和免疫功能。在妊娠第35天至第110天，将32只怀有双胎胎儿的湖羊母羊随机分为四组之一（每组n = 8）：100%美国国家研究委员会（NRC）推荐的营养需求（CON）、50%NRC推荐量（RES）、50%NRC推荐量并补充20g/天的RP-Arg（RES+ARG）以及50%NRC推荐量并补充5g/天的NCG（RES+NCG）。与CON组母羊相比，RES组的髓质厚度增加（P<0.05），但与RES组母羊相比，RES+ARG组和RES+NCG组的髓质厚度均降低（P<0.05）。与CON组相比，RES+ARG组和RES+NCG组的超氧化物歧化酶和谷胱甘肽过氧化物酶活性或丙二醛水平无差异（P>0.05）。与CON组母羊相比，RES组胎儿脐带血中IgA、白细胞介素（IL）-1β和IL-10的浓度降低（P<0.05），但RES+ARG组和RES+NCG组均升高（P<0.05）。与CON组母羊相比，RES组中Bax、Fas和p53 mRNA的表达增加（P<0.05），但RES+ARG组和RES+NCG组均降低（P>0.05）。这些结果表明，日粮中添加NCG和RP-Arg有助于减轻子宫内生长受限对胎儿胸腺发育和免疫功能的负面影响。
