Gross T S, Plante C, Thatcher W W, Hansen P J, Helmer S D, Putney D J
Dairy Science Department, University of Florida, Gainesville 32611.
Biol Reprod. 1988 Nov;39(4):977-87. doi: 10.1095/biolreprod39.4.977.
The conceptus is believed to produce factors that regulate endometrial function and prevent luteolysis during early pregnancy. Endometrial tissues were collected from cyclic (n = 8) and pregnant (n = 2) cows at Day 17 post-estrus and cultured for 24 and 48 h with bovine conceptus secretory proteins (bCSP) (0%, 10%, 100%), where the amount of protein produced by a bovine conceptus during 24 h of culture is 100%. Incorporation of [3H]leucine into secreted proteins was determined and examined qualitatively by trichloroacetic acid precipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography. Levels of an intracellular endometrial inhibitor of prostaglandin synthesis were determined with a cotyledonary microsomal test system. Treatment with 10% and 100% bCSP reduced incorporation of [3H]leucine into secreted proteins. However, bCSP selectively induced two secreted proteins (13 and 10 kDa) from endometrium of cyclic cows. Prostaglandin F (PGF) secretion was decreased by bCSP treatment while prostaglandin E2 secretion was unaltered. An intracellular endometrial inhibitor of prostaglandin synthesis was induced by bCSP; synthesis of PGF by the cotyledonary prostaglandin-generating system was decreased when incubated with cytosol of endometrium treated with bCSP, but unaltered by cytosol from control tissues. In conclusion, products produced by the bovine conceptus are capable of regulating endometrial protein and prostaglandin biosynthesis in a fashion that could act to prevent luteolysis in vivo and provide endometrial secretory products for embryonic development.
人们认为，孕体在妊娠早期会产生调节子宫内膜功能并防止黄体溶解的因子。在发情后第17天，从处于发情周期（n = 8）和怀孕（n = 2）的母牛身上采集子宫内膜组织，并用牛孕体分泌蛋白（bCSP）（0%、10%、100%）培养24小时和48小时，其中牛孕体在24小时培养期间产生的蛋白量为100%。通过三氯乙酸沉淀、十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳和荧光显影法测定并定性检测[3H]亮氨酸掺入分泌蛋白的情况。用子叶微粒体测试系统测定细胞内子宫内膜前列腺素合成抑制剂的水平。用10%和100%的bCSP处理可减少[3H]亮氨酸掺入分泌蛋白。然而，bCSP选择性地诱导了发情周期母牛子宫内膜产生两种分泌蛋白（13 kDa和10 kDa）。bCSP处理可降低前列腺素F（PGF）的分泌，而前列腺素E2的分泌未改变。bCSP诱导了细胞内子宫内膜前列腺素合成抑制剂的产生；当与用bCSP处理的子宫内膜胞质溶胶一起孵育时，子叶前列腺素生成系统合成PGF的能力降低，但对照组织的胞质溶胶未使其改变。总之，牛孕体产生的产物能够调节子宫内膜蛋白和前列腺素的生物合成，其方式可能有助于在体内防止黄体溶解，并为胚胎发育提供子宫内膜分泌产物。
