Dept. of Food and Nutrition, Chung-Ang Univ., Anseoung-si, Gyeonggi-do, 456-756, Korea.
Food and Nutrition in Home Economics, Korea Natl. Open Univ., 169 Dongsung-Dong, Jongno-Gu, Seoul, 110-791, Korea.
J Food Sci. 2020 Mar;85(3):639-646. doi: 10.1111/1750-3841.14981. Epub 2020 Feb 20.
Peanut sprouts are a functional food material rich in phytochemicals, including trans-resveratrol. This study aimed to optimize the recovery of trans-resveratrol from peanut sprouts using a combination of peanut varieties and sawdust medium through accelerated solvent extraction (ASE) and the response surface method (RSM). We also aimed to determine the antioxidant activity of this trans-resveratrol extract. Optimal fermentation periods of sawdust and peanut variety for cultivating peanut sprouts were determined on the basis of trans-resveratrol content via high-performance liquid chromatography. The extraction variables temperature, static time, and ethanol concentration were used to create a 20-sample set fit to a second-order polynomial equation through multiple regression analysis (R = 0.8787, P < 0.01). Trans-resveratrol content (19.62 ± 2.33 µg/g) peaked in the Palgwang variety cultured in sawdust medium fermented for 45 days. Optimal conditions for ASE were determined regarding the extraction temperature (90.29 °C), static time (3.95 min), and solvent (81.54% EtOH/water), and the predicted trans-resveratrol content under optimal conditions was 30.23 µg/g. Sawdust medium was more effective in increasing the trans-resveratrol content than conventional hydroponics, and the optimized process of combining fermented sawdust cultivation for harvesting peanut sprouts with ASE has potential as an efficient method of obtaining mass quantities of trans-resveratrol from peanut sprouts with improved nutritional and functional properties. PRACTICAL APPLICATION: This study showed that sawdust medium is more effective than hydroponics in increasing the trans-resveratrol content in peanut sprouts. The recovery of trans-resveratrol from peanut sprouts and its antioxidant activity were optimized via accelerated solvent extraction (ASE) and the response surface methodology (RSM). The optimized process of combining fermented sawdust cultivation for harvesting peanut sprouts with ASE potentially provides an efficient method to obtain mass quantities of trans-resveratrol from peanut sprouts with improved nutritional and functional properties.
花生芽是一种富含植物化学物质的功能性食品材料，其中包括反式白藜芦醇。本研究旨在通过加速溶剂萃取（ASE）和响应面法（RSM），结合花生品种和木屑培养基优化反式白藜芦醇的回收。我们还旨在确定该反式白藜芦醇提取物的抗氧化活性。通过高效液相色谱法测定反式白藜芦醇含量，确定木屑和花生品种的最佳发酵期，以培养花生芽。通过多元回归分析（R = 0.8787，P < 0.01），使用提取温度、静态时间和乙醇浓度等变量创建了 20 个样品集拟合二阶多项式方程。Palgwang 品种在发酵 45 天的木屑培养基中培养时，反式白藜芦醇含量（19.62 ± 2.33 µg/g）最高。针对 ASE 提取温度（90.29°C）、静态时间（3.95 分钟）和溶剂（81.54% EtOH/水），确定了最佳条件，最佳条件下预测的反式白藜芦醇含量为 30.23 µg/g。木屑培养基比传统水培法更有效地提高了花生芽中的反式白藜芦醇含量，结合发酵木屑培养收获花生芽和 ASE 的优化工艺，为从具有改善的营养和功能特性的花生芽中获得大量反式白藜芦醇提供了一种有效的方法。
本研究表明，与水培法相比，木屑培养基更有效地提高了花生芽中的反式白藜芦醇含量。通过加速溶剂萃取（ASE）和响应面法（RSM）优化了从花生芽中回收反式白藜芦醇及其抗氧化活性。结合发酵木屑培养收获花生芽和 ASE 的优化工艺为从具有改善的营养和功能特性的花生芽中获得大量反式白藜芦醇提供了一种有效的方法。
