Aquatic Ecology and Toxicology Section, Centre for Organismal Studies, University of Heidelberg, Im Neuenheimer Feld 504, Heidelberg, D-69120, Germany.
Aquatic Ecology and Toxicology Section, Centre for Organismal Studies, University of Heidelberg, Im Neuenheimer Feld 504, Heidelberg, D-69120, Germany.
Ecotoxicol Environ Saf. 2020 Apr 1;192:110330. doi: 10.1016/j.ecoenv.2020.110330. Epub 2020 Feb 17.
Zebrafish (Danio rerio) early life-stages are increasingly gaining attention as an alternative model in both human and environmental toxicology. Whereas there is amble knowledge about the transcription of various cytochrome P450 isoforms, the level of information about functional implications is still limited. This study investigated the development of CYP2-dependent 7-methoxycoumarin-O-demethylase (MCOD) activity throughout the early zebrafish development from 5 to 118 h post-fertilization (hpf) via confocal laser scanning microscopy. Results demonstrate that zebrafish embryos exhibit constitutive MCOD activity from as early as 5.5 hpf. Characteristic spatiotemporal patterns were documented with MCOD activities localized in several tissues and organs, namely the cardiovascular system, the brain, the digestive system, and the urinary tract. The study thereby contributes to a better understanding of the development and functional role of CYP enzymes in zebrafish early life-stages.
斑马鱼（Danio rerio）早期生命阶段作为人类和环境毒理学的替代模型，越来越受到关注。虽然人们对各种细胞色素 P450 同工型的转录有充分的了解，但关于功能影响的信息水平仍然有限。本研究通过共聚焦激光扫描显微镜，研究了 CYP2 依赖性 7-甲氧基香豆素-O-脱甲基酶（MCOD）活性在受精后 5 至 118 小时（hpf）整个早期斑马鱼发育过程中的发展情况。结果表明，斑马鱼胚胎早在 5.5 hpf 就表现出组成型 MCOD 活性。特征时空模式记录了 MCOD 活性定位于几个组织和器官，即心血管系统、大脑、消化系统和泌尿系统。该研究有助于更好地了解 CYP 酶在斑马鱼早期生命阶段的发育和功能作用。
