INRAE, Université Clermont Auvergne, Vetagro Sup, UMRH, 63122 Saint-Genès-Champanelle, France.
Laboratoire de Spectrométrie de Masse BioOrganique (LSMBO), IPHC UMR 7178, CNRS, Université de Strasbourg, 67000 Strasbourg, France.
Food Chem. 2020 Jul 1;317:126376. doi: 10.1016/j.foodchem.2020.126376. Epub 2020 Feb 10.
We and others have identified biomarker candidates of tenderness or marbling, two major attributes of bovine meat-eating qualities for consumers' satisfaction. In this study, Reverse Phase Protein Arrays (RPPA) and targeted mass spectrometry assays using Parallel Reaction Monitoring (PRM) were developed to test whether 10 proteins pass the sequential qualification and verification steps of the challenging biomarker discovery pipeline. At least MYH1, TPI1, ALDH1A1 and CRYAB were qualified by RPPA or PRM as being differentially abundant according to marbling values of longissimus thoracis and semimembranosus muscles. Significant mathematical relationships between the individual abundance of each of the four proteins and marbling values were verified by linear or logistic regressions. Four proteins, TNNT1, MDH1, PRDX6 and ENO3 were qualified and verified for tenderness, and the abundance of MDH1 explained 49% of the tenderness variability. The present PRM and RPPA results pave the way for development of useful meat industrial multiplex-proteins assays.
我们和其他人已经确定了嫩度或大理石花纹的生物标志物候选物，这是牛肉食用品质的两个主要属性，可满足消费者的满意度。在这项研究中，开发了反相蛋白阵列（RPPA）和使用平行反应监测（PRM）的靶向质谱分析，以测试是否有 10 种蛋白质通过具有挑战性的生物标志物发现管道的连续资格和验证步骤。至少 MYH1、TPI1、ALDH1A1 和 CRYAB 通过 RPPA 或 PRM 被证明根据背最长肌和半膜肌的大理石花纹值存在差异丰度。通过线性或逻辑回归验证了这四种蛋白质的每种蛋白质的个体丰度与大理石花纹值之间的显著数学关系。TNNT1、MDH1、PRDX6 和 ENO3 被鉴定为嫩度的标志物，MDH1 的丰度解释了 49%的嫩度变异性。本 PRM 和 RPPA 的结果为开发有用的肉类工业多蛋白分析铺平了道路。
