Mertowski Sebastian, Grywalska Ewelina, Gosik Krzysztof, Smarz-Widelska Iwona, Hymos Anna, Dworacki Grzegorz, Niedźwiedzka-Rystwej Paulina, Drop Bartłomiej, Roliński Jacek, Załuska Wojciech
Department of Clinical Immunology and Immunotherapy, Medical University of Lublin, Lublin 20-093, Poland.
Department of Immunology, St. John's Cancer Centre, Lublin 20-090, Poland.
J Clin Med. 2020 Feb 17;9(2):541. doi: 10.3390/jcm9020541.
Toll-like receptor (TLR) signaling may be involved in autoimmune kidney disorders and has been implicated in proliferative and non-proliferative glomerulonephritis (PGN and NPGN). In this study, we investigated the expression of TLR2 on T and B lymphocytes in relation to selected clinical parameters in patients with PGN and NPGN. We collected peripheral blood from the ulnar vein of patients with PGN ( = 15) or NPGN ( = 22) and healthy volunteers ( = 20). The percentage of peripheral blood mononuclear cells expressing TLR2 was determined with flow cytometry. TLR2 expression on T and B lymphocytes was increased in PGN patients compared with NPGN patients and controls ( ≤ 0.001). In patients with PGN, TLR2 expression correlated negatively with the serum concentrations of IgG and albumin and positively with urine protein excretion. Receiver operating characteristic (ROC) analysis indicated that TLR2 expression is a highly specific marker to distinguish PGN patients from NPGN patients and controls, especially on CD4+ T lymphocytes. Its use as a non-invasive marker of disease should be further investigated.
Toll样受体（TLR）信号传导可能参与自身免疫性肾脏疾病，并与增殖性和非增殖性肾小球肾炎（PGN和NPGN）有关。在本研究中，我们调查了PGN和NPGN患者T和B淋巴细胞上TLR2的表达与选定临床参数的关系。我们从PGN患者（n = 15）、NPGN患者（n = 22）和健康志愿者（n = 20）的尺静脉采集外周血。用流式细胞术测定表达TLR2的外周血单个核细胞百分比。与NPGN患者和对照组相比，PGN患者T和B淋巴细胞上的TLR2表达增加（P≤0.001）。在PGN患者中，TLR2表达与IgG和白蛋白的血清浓度呈负相关，与尿蛋白排泄呈正相关。受试者工作特征（ROC）分析表明，TLR2表达是区分PGN患者与NPGN患者和对照组的高度特异性标志物，尤其是在CD4+T淋巴细胞上。应进一步研究将其用作疾病的非侵入性标志物。
