State Key Laboratory of Crop Stress Biology in Arid Areas, Yangling 712100, China.
College of Agronomy, Northwest A&F University, Yangling 712100, China.
Int J Mol Sci. 2020 Feb 17;21(4):1345. doi: 10.3390/ijms21041345.
The use of herbicides is an effective and economic way to control weeds, but their availability for rapeseed is limited due to the shortage of herbicide-resistant cultivars in China. The single-point mutation in the () gene can lead to AHAS-inhibiting herbicide resistance. In this study, the inheritance and molecular characterization of the tribenuron-methyl (TBM)-resistant rapeseed ( L.) mutant, are performed. Results indicated that TBM-resistance of was controlled by one dominant allele at a single nuclear gene locus. The novel substitution of cytosine with thymine at position 544 in was identified in , leading to the alteration of proline with serine at position 182 in BnAHAS1. The TBM-resistance of was approximately 100 times that of its wild-type , and also showed cross-resistance to bensufuron-methyl and monosulfuron-ester sodium. The transgenic exhibited higher TBM-resistance than that of its wild-type, which confirmed that was responsible for the herbicide resistance of . Simultaneously, an allele-specific marker was developed to quickly distinguish the heterozygous and homozygous mutated alleles . In addition, a method for the fast screening of TBM-resistant plants at the cotyledon stage was developed. Our research identified and molecularly characterized one novel mutative allele in and laid a foundation for developing herbicide-resistant rapeseed cultivars.
除草剂的使用是控制杂草的一种有效且经济的方法，但由于中国缺乏抗除草剂品种，油菜对除草剂的可用性有限。（）基因的单点突变可导致 AHAS 抑制剂除草剂抗性。本研究对啶磺草胺（TBM）抗油菜（）突变体的遗传和分子特征进行了研究。结果表明，的 TBM 抗性由单个核基因座上的一个显性等位基因控制。在，发现 544 位的胞嘧啶被胸腺嘧啶取代，导致 BnAHAS1 第 182 位脯氨酸被丝氨酸取代，这是一个新的突变。与野生型相比，的 TBM 抗性约为 100 倍，对苯磺隆和单嘧磺酯钠盐也表现出交叉抗性。转 基因的 表现出比其野生型更高的 TBM 抗性，这证实了是其对除草剂产生抗性的原因。同时，开发了一个等位基因特异性标记，以快速区分杂合和纯合突变等位基因。此外，还开发了一种在子叶阶段快速筛选 TBM 抗性植物的方法。本研究在油菜中鉴定并分子特征化了一个新的突变 等位基因，为开发抗除草剂油菜品种奠定了基础。
