Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China.
College of Pharmacy, Jinan University, Guangzhou 510632, China.
J Agric Food Chem. 2020 Mar 11;68(10):3214-3219. doi: 10.1021/acs.jafc.9b08303. Epub 2020 Mar 2.
Four terpene synthases for the biosynthesis of volatile terpenoids were identified from the transcriptome of L. flowers, including SchTPS1, SchTPS2, SchTPS3, and SchTPS4. Their functions were characterized by synthetic biology approaches in and in vitro enzymatic assays. SchTPS1, SchTPS2, and SchTPS3 are guaiene synthases, while SchTPS4 is an (,)-geranyl linalool synthase. Next, SchTPS1 and α-guaiene 2-oxidase VvSTO2 were co-expressed in to reconstruct the biosynthetic pathway of (-)-rotundone, which is a unique aroma compound in fruits, vegetables, and wines. This is the first report for the construction of a (-)-rotundone-producing microbial platform.
从 L. 花的转录组中鉴定出了 4 种用于生物合成挥发性萜类化合物的萜烯合酶，包括 SchTPS1、SchTPS2、SchTPS3 和 SchTPS4。通过 和体外酶促测定的合成生物学方法对它们的功能进行了表征。SchTPS1、SchTPS2 和 SchTPS3 是愈创木烯合酶，而 SchTPS4 是（,）-香叶基芳樟醇合酶。接下来，SchTPS1 和 α-愈创木烯 2-氧化酶 VvSTO2 在 中共同表达，以重建（-）-罗通定的生物合成途径，（-）-罗通定是水果、蔬菜和葡萄酒中独特的香气化合物。这是构建（-）-罗通定生产微生物平台的首次报道。
