College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, Guangdong, People's Republic of China.
Biol Trace Elem Res. 2020 Dec;198(2):636-643. doi: 10.1007/s12011-020-02076-0. Epub 2020 Feb 20.
The purpose of this research was to investigate whether copper (Cu) exposure could induce apoptosis via endoplasmic reticulum stress (ERS) in skeletal muscle of broilers. A total of 240 one-day-old chickens were randomly divided into four groups by free access; the diets are as follows: control diet (Cu 11 mg/kg, control group) and high level of Cu diets (Cu 110 mg/kg, group I; Cu 220 mg/kg, group II; Cu 330 mg/kg, group III). The skeletal muscle tissues were collected on day 49 for further examination. The content of Cu, histopathology, and the expression levels of the genes and proteins related to ERS and apoptosis were detected. Results showed that the Cu levels in skeletal muscle were increased in a dose-dependent manner. Meanwhile, the spaces between the muscle fibers were wider with the increase of Cu content, and the myolysis was observed in group III. Besides, the mRNA expression levels of GRP78, GRP94, eIF2α, ATF6, XBP1, CHOP, Caspase-12, and Caspase3 were markedly increased in treated groups compared with control group, and the protein expression levels of GRP78, Caspase3, Active-Caspase3 and JNK were significantly elevated with the increase of dietary Cu. In summary, these findings suggested that Cu could induce apoptosis through ERS in skeletal muscle of broilers.
本研究旨在探讨铜（Cu）暴露是否会通过内质网应激（ERS）诱导肉鸡骨骼肌细胞凋亡。将 240 只 1 日龄雏鸡自由采食，随机分为 4 组，日粮分别为：基础日粮（Cu11mg/kg，对照组）和高铜日粮（Cu110mg/kg，Ⅰ组；Cu220mg/kg，Ⅱ组；Cu330mg/kg，Ⅲ组）。第 49 天采集骨骼肌组织，检测 Cu 含量、组织病理学变化以及与 ERS 和凋亡相关的基因和蛋白的表达水平。结果表明，骨骼肌中 Cu 含量呈剂量依赖性增加。随着 Cu 含量的增加，肌纤维间隙变宽，在Ⅲ组中观察到肌溶解。与对照组相比，处理组的 GRP78、GRP94、eIF2α、ATF6、XBP1、CHOP、Caspase-12 和 Caspase3 的 mRNA 表达水平显著增加，GRP78、Caspase3、Active-Caspase3 和 JNK 的蛋白表达水平也随日粮中 Cu 含量的增加而显著升高。综上所述，这些结果表明 Cu 可能通过 ERS 诱导肉鸡骨骼肌细胞凋亡。
