Tanaka K, Gotoh F, Ishihara N, Gomi S, Takashima S, Mihara B
Department of Neurology, School of Medicine, Keio University, Tokyo, Japan.
Brain Res Bull. 1988 Oct;21(4):693-700. doi: 10.1016/0361-9230(88)90210-9.
Quantitative in-vitro autoradiographic study was performed to localize two prominent second-messenger systems (the adenylate cyclase and phosphoinositide systems) in the normal gerbil brain. [3H] Forskolin and [3H] phorbol 12, 13-dibutyrate (PDBu) were used to identify the regional distribution of adenylate cyclase and protein kinase C, respectively. The localization of the forskolin binding was not uniform, being particularly concentrated in the striatum, the accumbens nucleus, the olfactory tubercle, the substantia nigra, the CA3 region of the hippocampus and the molecular layer of the cerebellum. On the other hand, the PDBu binding was rather uniform, although the superficial layer of the cerebral neocortices, the strata oriens of the CA1 region of the hippocampus and the molecular layer of the cerebellum showed relatively dense binding. Quantitative autoradiography of the second-messenger systems in the brain is expected to provide important information concerning the role of neurotransmitters in the pathophysiology of various conditions.
进行了定量体外放射自显影研究，以定位正常沙鼠脑中两种主要的第二信使系统（腺苷酸环化酶和磷酸肌醇系统）。分别使用[3H]福斯高林和[3H]佛波醇12,13 - 二丁酸酯（PDBu）来确定腺苷酸环化酶和蛋白激酶C的区域分布。福斯高林结合的定位并不均匀，特别集中在纹状体、伏隔核、嗅结节、黑质、海马体的CA3区和小脑分子层。另一方面，PDBu结合相当均匀，尽管大脑新皮质的表层、海马体CA1区的 Oriens层和小脑分子层显示出相对密集的结合。大脑中第二信使系统的定量放射自显影有望提供有关神经递质在各种疾病病理生理学中作用的重要信息。
