Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan; Department of Urology, National Taiwan University Hospital, Taipei, Taiwan.
Department of Urology, National Taiwan University Hospital, Taipei, Taiwan.
J Formos Med Assoc. 2020 Nov;119(11):1673-1683. doi: 10.1016/j.jfma.2019.12.015. Epub 2020 Feb 17.
BACKGROUND/PURPOSE: The inflammatory milieu has been firmly established to affect cancer progression. However, the connection between natural killer (NK) cells and prostate cancer (PCa) has not been elucidated.
Prospective data on NK cell activity (NKA) and NK cell subset distribution patterns were evaluated from 51 patients treated with robot-assisted laparoscopic radical prostatectomy. Whole-blood samples were collected from patients preoperatively and 4-6 weeks postoperatively. The samples were subjected to NKA tests, NK cell number counts, determination of the NKG2D (activating receptor of NK cells), NKG2A (inhibiting receptor), and other surface markers. All the analyses were compared to the clinicopathological characteristics of patients. NKA was estimated by measuring interferon-γ (IFN-γ) levels after stimulation of the peripheral blood with PROMOCA™, which specifically stimulates the release of IFN-γ from NK cells.
NKA was lower in patients with PCa than in healthy participants (484.66 vs. 1550 pg/mL). A paired comparison revealed significantly higher NKA postoperatively than preoperatively (1054 vs. 484.66 pg/mL; p = 0.011). Patients with negative surgical margins exhibited significantly higher postoperative NKA and NKA ratio (postoperative NKA/preoperative NKA) than those with positive margins (557 vs. 1921 pg/mL, p < 0.001; 3.6 vs. 1.59, p = 0.024). However, there was no difference in the postoperative NK cell number or the CD56/CD16/CD3 or CD56/CD16/CD3 cell numbers between the negative and positive margin groups. Postoperative NKA was significantly higher in lower-stage (1/2) than in higher-stage (3/4) PCa (1365 vs. 594 pg/mL, p = 0.014).
NKA was significantly higher postoperatively than preoperatively. Patients with positive surgical margins had lower postoperative NKA than those with negative margins. Lower postoperative NKA was also observed in higher-stage PCa. NKA could be used as a supplemental marker for detecting the remaining tumor cells after prostatectomy in combination of PSA.
背景/目的：炎症环境已被确定会影响癌症的进展。然而，自然杀伤 (NK) 细胞与前列腺癌 (PCa) 之间的联系尚未阐明。
对 51 例接受机器人辅助腹腔镜根治性前列腺切除术的患者的 NK 细胞活性 (NKA) 和 NK 细胞亚群分布模式的前瞻性数据进行了评估。从患者术前和术后 4-6 周采集全血样本。对样本进行 NKA 测试、NK 细胞计数、NKG2D（NK 细胞的激活受体）、NKG2A（抑制受体）和其他表面标志物的测定。将所有分析结果与患者的临床病理特征进行比较。NKA 通过用 PROMOCA™刺激外周血来测量干扰素-γ (IFN-γ) 水平来估计，PROMOCA™ 特异性刺激 NK 细胞释放 IFN-γ。
与健康参与者相比，PCa 患者的 NKA 较低（484.66 比 1550 pg/mL）。配对比较显示术后 NKA 明显高于术前（1054 比 484.66 pg/mL；p = 0.011）。阴性切缘患者的术后 NKA 和 NKA 比值（术后 NKA/术前 NKA）明显高于阳性切缘患者（557 比 1921 pg/mL，p < 0.001；3.6 比 1.59，p = 0.024）。然而，阴性和阳性切缘组之间的术后 NK 细胞数或 CD56/CD16/CD3 或 CD56/CD16/CD3 细胞数无差异。低分期（1/2）患者的术后 NKA 明显高于高分期（3/4）PCa（1365 比 594 pg/mL，p = 0.014）。
术后 NKA 明显高于术前。阳性切缘患者的术后 NKA 低于阴性切缘患者。较高分期的 PCa 患者的术后 NKA 也较低。NKA 可与 PSA 联合作为前列腺切除术后检测残留肿瘤细胞的辅助标志物。
