罗非鱼 DDX41 在 IFN 调控中的分子与功能特征。
Molecular and functional characterization of tilapia DDX41 in IFN regulation.
机构信息
Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, China; State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Fishery, Guangdong Ocean University, Zhanjiang, 524025, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen, 518120, China; Shenzhen Dapeng New District Science and Technology Innovation Service Center, Shenzhen, 518120, China.
Shenzhen Institute of Guangdong Ocean University, Shenzhen, 518120, China; College of Fishery, Guangdong Ocean University, Zhanjiang, 524025, China; Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Shenzhen, 518120, China.
出版信息
Fish Shellfish Immunol. 2020 Apr;99:386-391. doi: 10.1016/j.fsi.2020.02.031. Epub 2020 Feb 17.
DEAD-box helicase 41 (DDX41) is a key cytosolic DNA sensor playing critical roles in the regulation of type I IFN responses, and their functions have been well-characterized in mammals. However, little information is available regarding the function of fish DDX41. In this study, a DDX41 gene, named On-DDX41, was identified in Nile tilapia, Oreochromis niloticus. The predicted protein of On-DDX41 contains several structural features known in DDX41, including conserved DEADc and HELICc domains, and a conserved sequence "Asp-Glu-Ala-Asp (D-E-A-D)". On-DDX41 gene was constitutively expressed in all tissues examined, with the highest expression level observed in liver and muscle, and was inducible after poly(I:C) stimulation. Moreover, the overexpression of On-DDX41 can elicit a strong activation of both zebrafish IFN1 and IFN3 promoter in fish cells treated with poly(dA:dT). The present study thus contributes to a better understanding of the functional properties of DDX41 in fish.
DEAD-box 解旋酶 41（DDX41）是一种关键的细胞质 DNA 传感器，在调节 I 型 IFN 反应中发挥着重要作用，其功能在哺乳动物中得到了很好的描述。然而，关于鱼类 DDX41 的功能信息却很少。在本研究中，我们在尼罗罗非鱼（Oreochromis niloticus）中鉴定了一个 DDX41 基因，命名为 On-DDX41。On-DDX41 蛋白含有多个已知的 DDX41 结构特征，包括保守的 DEADc 和 HELICc 结构域，以及一个保守序列 "Asp-Glu-Ala-Asp (D-E-A-D)"。On-DDX41 基因在所有检测到的组织中均呈组成型表达，在肝脏和肌肉中表达水平最高，并且在 poly(I:C)刺激后可诱导表达。此外，在用 poly(dA:dT)处理的鱼类细胞中，过表达 On-DDX41 可以强烈激活斑马鱼 IFN1 和 IFN3 启动子。因此，本研究有助于更好地理解 DDX41 在鱼类中的功能特性。
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