Urushihara H, Habata Y, Yanagisawa K
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Cell Differ Dev. 1988 Nov;25(2):81-7. doi: 10.1016/0922-3371(88)90001-9.
The molecular mechanism of sexual cell fusion in Dictyostelium discoideum was studied using the heterothallic strains HM1 and NC4. Monovalent antibodies (Fab) prepared from rabbit antiserum against a crude membrane preparation of fusion-competent HM1 cells inhibited fusion between HM1 and NC4 cells. Six out of 43 antigenic proteins were found in fusion-competent HM1 cells but not in fusion-incompetent cells. Among them, only one protein with a molecular mass of 70 kDa was able to neutralize the fusion-inhibiting activity of Fab, suggesting its possible participation in sexual cell fusion.
利用异宗配合菌株HM1和NC4研究了盘基网柄菌有性细胞融合的分子机制。用兔抗融合能力强的HM1细胞粗膜制剂的抗血清制备的单价抗体（Fab）抑制了HM1和NC4细胞之间的融合。在融合能力强的HM1细胞中发现了43种抗原蛋白中的6种，而在融合能力弱的细胞中未发现。其中，只有一种分子量为70 kDa的蛋白质能够中和Fab的融合抑制活性，表明其可能参与有性细胞融合。
