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Isolation and characterization of a sea urchin hsp 70 gene segment.

作者信息

Sconzo G, La Rosa M, La Farina M, Roccheri M C, Oliva D, Giudice G

机构信息

Dipartimento di Biologia Cellulare e dello Sviluppo, CNR, Palermo, Italy.

出版信息

Cell Differ. 1988 Jul;24(2):97-104. doi: 10.1016/0045-6039(88)90061-9.

DOI:10.1016/0045-6039(88)90061-9
PMID:3208287
Abstract

Three clones containing Paracentrotus lividus sea urchin DNA sequences which cross-hybridize to Drosophila heat shock protein (hsp) 70 gene were isolated. The sequence arrangements in the three cloned DNA inserts were compared by restriction and cross-hybridization analysis. The results showed that they contain four different genes related to one Drosophila hsp 70 gene. One of these genes was subcloned, and two of the isolated fragments were shown to hybridize to genomic DNA and to RNA from heat-treated sea urchin embryo.

摘要

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