Idroes Rinaldi, Idroes Ghazi Mauer, Suhendra Rivansyah
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Kopelma Darussalam, Banda Aceh 23111, Indonesia.
Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Kopelma Darussalam, Banda Aceh 23111, Indonesia.
Heliyon. 2020 Feb 12;6(2):e03302. doi: 10.1016/j.heliyon.2020.e03302. eCollection 2020 Feb.
The precise determination of the dead time is essential in a chromatography system as it is a primary parameter for the determination of other secondary parameters such as the adjusted retention time, relative retention time, retention factor and retention index. Several of the indirect methods used for the determination of the dead time in this study were iteration, nonlinear, spreadsheet and statistics methods, which were implemented using the ANSI C programming language. The calculation of each method was tested with temperature and column variations for measuring the retention time of a n-alkane homologous series and accuracy analysis of each mathematical method (indirect method) to the marker substance (direct method). Changes in the temperature and column variations (type, polarity and column length) affected the calculation of the dead time values but did not affect its accuracy. The value of the dead time generated by the non-linear method was relatively high, with errors above 10%, while the other methods utilized are quite good with errors below 8% regardless of the column and temperature variations.
在色谱系统中，准确测定死时间至关重要，因为它是测定其他次要参数（如调整保留时间、相对保留时间、保留因子和保留指数）的主要参数。本研究中用于测定死时间的几种间接方法包括迭代法、非线性法、电子表格法和统计法，这些方法是使用ANSI C编程语言实现的。每种方法的计算都针对温度和色谱柱变化进行了测试，以测量正构烷烃同系物的保留时间，并对每种数学方法（间接法）相对于标记物质（直接法）进行准确性分析。温度和色谱柱变化（类型、极性和柱长）会影响死时间值的计算，但不影响其准确性。非线性方法产生的死时间值相对较高，误差超过10%，而其他使用的方法无论色谱柱和温度如何变化，误差均低于8%，效果相当不错。
