鉴定鼻咽癌转移中包含 miR-106b、miR-17、miR-20b、miR-18a 和 miR-93 的新型 microRNA 谱。

Identification of a novel microRNA profile including miR-106b, miR-17, miR-20b, miR-18a and miR-93 in the metastasis of nasopharyngeal carcinoma.

机构信息

Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Chongqing Cancer Institute and Chongqing Cancer Hospital, Chongqing, China.

Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.

出版信息

Cancer Biomark. 2020;27(4):533-539. doi: 10.3233/CBM-190601.

DOI:10.3233/CBM-190601

PMID:32083569

Abstract

BACKGROUND

Metastasis often leads to poor prognosis in nasopharyngeal carcinoma (NPC) patients. Evidence has indicated the important roles of microRNA (miRNA) in cancer metastasis. The aim of this study was to identify and verify the key miRNAs that might be involved in the development of NPC metastasis.

METHODS

Microarray data were obtained and analyzed to screen the differentially expressed miRNAs (DEMs) between NPC tissues with metastasis and those without metastasis. The target genes of the DEMs were predicted and their functions were annotated. Then, candidate hub genes were screened out through protein-protein interaction analysis, and the key miRNAs were identified. Afterwards, the expression levels of the key miRNAs were assessed by qRT-PCR based on an in vitro model.

RESULTS

A total of 22 DEMs were screened out, and 616 target genes were predicted. Gene Ontology (GO) and pathway enrichment analysis showed that the target genes may be enriched in a diversity of GO terms and signaling pathways. Among them, eleven hub genes were identified, such as PTEN, KAT2B, CCND1, STAT3, and MAP3K5. Moreover, a five-miRNA profile (miR-106b, miR-17, miR-20b, miR-18a and miR-93) was identified and their expression levels were tested to be up-regulated in high-metastatic NPC cells relative to low-metastatic ones.

CONCLUSION

The present study revealed that five miRNAs (miR-106b, miR-17, miR-20b, miR-18a and miR-93) and several hub genes such as PTEN, KAT2B, CCND1, STAT3, and MAP3K5, might play critical roles in the development of NPC metastasis. Future investigations are needed to confirm the results.

摘要

背景

转移常导致鼻咽癌（NPC）患者预后不良。有证据表明 microRNA（miRNA）在癌症转移中起重要作用。本研究旨在鉴定和验证可能参与 NPC 转移发展的关键 miRNA。

方法

获取并分析微阵列数据，以筛选有转移和无转移 NPC 组织之间差异表达的 miRNA（DEM）。预测 DEM 的靶基因并对其功能进行注释。然后，通过蛋白质-蛋白质相互作用分析筛选候选枢纽基因，并确定关键 miRNA。随后，基于体外模型通过 qRT-PCR 评估关键 miRNA 的表达水平。

结果

共筛选出 22 个 DEM，预测出 616 个靶基因。基因本体论（GO）和通路富集分析表明，靶基因可能富集在多种 GO 术语和信号通路中。其中，确定了 11 个枢纽基因，如 PTEN、KAT2B、CCND1、STAT3 和 MAP3K5。此外，鉴定了一个由五个 miRNA 组成的谱（miR-106b、miR-17、miR-20b、miR-18a 和 miR-93），并测试其表达水平在高转移性 NPC 细胞中相对低转移性细胞上调。