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琼脂糖天然胶电泳用于抗体鉴定。

Agarose native gel electrophoresis for characterization of antibodies.

机构信息

Alliance Protein Laboratories, a Division of KBI Biopharma, 6042 Cornerstone Court West, San Diego, CA 92121, United States of America.

Research and Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-shi, Ibaraki 318-0004, Japan.

出版信息

Int J Biol Macromol. 2020 May 15;151:885-890. doi: 10.1016/j.ijbiomac.2020.02.185. Epub 2020 Feb 19.

DOI:10.1016/j.ijbiomac.2020.02.185
PMID:32084469
Abstract

This study was conducted to evaluate applicability of the previously reported native agarose gel electrophoresis to the analysis of various monoclonal and polyclonal antibodies. Experiments were carried to test the electrophoresis system for characterization of different monoclonal antibodies and animal serum, analysis of expressed antibodies in cell culture and evaluation of antibody stability. An attempt to optimize the electrophoretic condition was made by adjusting the electrode buffer concentration, electrophoretic run time and agarose concentration.

摘要

本研究旨在评估先前报道的天然琼脂糖凝胶电泳在分析各种单克隆和多克隆抗体中的适用性。进行了实验以测试电泳系统用于表征不同的单克隆抗体和动物血清，分析细胞培养中表达的抗体以及评估抗体稳定性。通过调整电极缓冲液浓度、电泳运行时间和琼脂糖浓度来尝试优化电泳条件。

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Agarose native gel electrophoresis for characterization of antibodies.琼脂糖天然胶电泳用于抗体鉴定。
Int J Biol Macromol. 2020 May 15;151:885-890. doi: 10.1016/j.ijbiomac.2020.02.185. Epub 2020 Feb 19.
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Western blotting of native proteins from agarose gels.从琼脂糖凝胶中对天然蛋白质进行 Western 印迹分析。
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Do DNA gel electrophoretic mobilities extrapolate to the free-solution mobility of DNA at zero gel concentration?DNA凝胶电泳迁移率能否外推至凝胶浓度为零时DNA在自由溶液中的迁移率？
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